Primer set sequences used for PCR analysis
Gene . | Forward (5′-3′) . | Reverse (5′-3′) . |
|---|---|---|
| hHPRT | TGACACTGGCAAAACAATGCA | GGTCCTTTTCACCAGCAAGCT |
| mHPRT | TTGCTCGAGATGTCATGAAGGA | AGCAGGTCAGCAAAGAACTTATAG |
| CCR2 | CTTGGGAATGAGTAACTGTGTGA | GGAGAGATACCTTCGGAACTTCT |
| CCR3 | TGCAGGTGACTGAGGTGATTG | CGGAACCTCTCACCAACAAAG |
| CCR5 | GACTGTCAGCAGGAAGTGAGCAT | CTTGACGCCAGCTGAGCAA |
| CXCR3 | GCTGCTGTCCAGTGGGTTTT | AGTTGATGTTGAACAAGGCGC |
| IFNγ | ATAACTATTTTAACTCAAGTGGCATAGATGT | TCTGGCTCTGCAGGATTTTCA |
| IFNβ | GCTCCTGGAGCAGCTGAATG | TCCGTCATCTCCATAGGGATCTT |
| SRY | GTGTGGTCCCTGGGTGAGA | TGTAAGGCTTTTCCACCTGCAT |
Gene . | Forward (5′-3′) . | Reverse (5′-3′) . |
|---|---|---|
| hHPRT | TGACACTGGCAAAACAATGCA | GGTCCTTTTCACCAGCAAGCT |
| mHPRT | TTGCTCGAGATGTCATGAAGGA | AGCAGGTCAGCAAAGAACTTATAG |
| CCR2 | CTTGGGAATGAGTAACTGTGTGA | GGAGAGATACCTTCGGAACTTCT |
| CCR3 | TGCAGGTGACTGAGGTGATTG | CGGAACCTCTCACCAACAAAG |
| CCR5 | GACTGTCAGCAGGAAGTGAGCAT | CTTGACGCCAGCTGAGCAA |
| CXCR3 | GCTGCTGTCCAGTGGGTTTT | AGTTGATGTTGAACAAGGCGC |
| IFNγ | ATAACTATTTTAACTCAAGTGGCATAGATGT | TCTGGCTCTGCAGGATTTTCA |
| IFNβ | GCTCCTGGAGCAGCTGAATG | TCCGTCATCTCCATAGGGATCTT |
| SRY | GTGTGGTCCCTGGGTGAGA | TGTAAGGCTTTTCCACCTGCAT |