Table 1. TACI gene expression yields a... | American Society of Hematology

Table 1.

TACI gene expression yields a BAFF/APRIL receptor with functional binding capacity in HMCLs

	PCR					Binding of BAFF-murine CD8	Real-time RT-PCR TACI
HMCLs	BCMA	BAFF-R	TACI			Binding of BAFF-murine CD8	Real-time RT-PCR TACI	Affymetrix TACI	Affymetrix BCMA
XG-12	+	–	+	+	+	P	P
XG-13	+^*	+^*	+^*	+^*	+	P	P
XG-19	+	+	+	+	+	P	P
XG-20	+^*	+^*	+^*	+^*	+	P	P
RPM18226	+^*	+^*	+^*	+^*	+	P	P
LP1	+^*	+^*	+^*	+^*	+	P	P
XG-1	+^*	+^*	–^*	–^*	–	A	P
XG-3	+	–	–	–	–	A	P
XG-4	+	+	–	–	–	A	P
XG-5	+^*	–^*	–^*	–^*	–	A	P
XG-6	+^*	–^*	–^*	–^*	–	A	P
XG-7	+^*	+^*	–^*	–^*	–	A	P
XG-10	+	+	–	–	–	A	P
XG-11	+^*	–^*	–^*	–^*	–	A	P
XG-16	+	–	–	–	–	A	P
OPM2	+	+	–	–	–	A	P
SKMM	+	–	–	–	–	A	P

	PCR					Binding of BAFF-murine CD8	Real-time RT-PCR TACI
HMCLs	BCMA	BAFF-R	TACI			Binding of BAFF-murine CD8	Real-time RT-PCR TACI	Affymetrix TACI	Affymetrix BCMA
XG-12	+	–	+	+	+	P	P
XG-13	+^*	+^*	+^*	+^*	+	P	P
XG-19	+	+	+	+	+	P	P
XG-20	+^*	+^*	+^*	+^*	+	P	P
RPM18226	+^*	+^*	+^*	+^*	+	P	P
LP1	+^*	+^*	+^*	+^*	+	P	P
XG-1	+^*	+^*	–^*	–^*	–	A	P
XG-3	+	–	–	–	–	A	P
XG-4	+	+	–	–	–	A	P
XG-5	+^*	–^*	–^*	–^*	–	A	P
XG-6	+^*	–^*	–^*	–^*	–	A	P
XG-7	+^*	+^*	–^*	–^*	–	A	P
XG-10	+	+	–	–	–	A	P
XG-11	+^*	–^*	–^*	–^*	–	A	P
XG-16	+	–	–	–	–	A	P
OPM2	+	+	–	–	–	A	P
SKMM	+	–	–	–	–	A	P

Expression of BCMA, BAFF-R, and TACI mRNA was determined by RT-PCR in 17 HMCLs. TACI and BCMA expressions were also determined with Affymetrix microarrays. The probeset for BAFF-R was not available on the Affymetrix HG U133 A + B DNA microarrays. The expression of BAFF binding was determined by flow cytometry using biotinylated human BAFF-murine CD8 fusion protein and phycoerythrin-conjugated streptavidin.

+ indicates the presence of the receptor; –, the receptor's absence; P, present cell; and A, absent cell.

These data are from our previously published data²¹

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