Comparison of multiplex RT-PCR with RQ-PCR
Multiplex RT-PCR . | L/A ratio less than 1 . | L/A ratio greater than 1 . | Total . |
|---|---|---|---|
| ADAM29 | 55 | 0 | 55 |
| LPL | 0 | 34 | 34 |
| Doublet | 4 | 2 | 6 |
| Total | 59 | 36 | 95 |
Multiplex RT-PCR . | L/A ratio less than 1 . | L/A ratio greater than 1 . | Total . |
|---|---|---|---|
| ADAM29 | 55 | 0 | 55 |
| LPL | 0 | 34 | 34 |
| Doublet | 4 | 2 | 6 |
| Total | 59 | 36 | 95 |
Cases were scored as ADAM29 or LPL according to the type of the predominant PCR product obtained by multiplex RT-PCR. When 2 bands of roughly similar intensity were observed, they were qualified as doublet.