Recombinant OPN induces TNF-α in DCs from TLR-4 wild-type C3H/HeN and TLR-defective C3H/eJ mice
. | C3H/HeN, pg/mL ± SD . | C3H/eJ, pg/mL ± SD . |
|---|---|---|
| Unstimulated | 7.2 ± 2.3 | 9.8 ± 2 |
| LPS, 29.7 EU/mL | 25.2 ± 0 | 12.9 ± 3.1 |
| OPN, 0.5 μg/mL | 17.9 ± 1.8 | 40.9 ± 24.5 |
. | C3H/HeN, pg/mL ± SD . | C3H/eJ, pg/mL ± SD . |
|---|---|---|
| Unstimulated | 7.2 ± 2.3 | 9.8 ± 2 |
| LPS, 29.7 EU/mL | 25.2 ± 0 | 12.9 ± 3.1 |
| OPN, 0.5 μg/mL | 17.9 ± 1.8 | 40.9 ± 24.5 |
Bone marrow DCs from TLR-4 wild-type C3H/HeN and TLR-defective C3H/eJ mice were generated as previously described10 and stimulated by the indicated concentrations of LPS or OPN for 48 hours. TNF-α secretion in supernatants was analyzed by ELISA. Mean of duplicate measurements is given in pg/mL ± SD. Results from one representative experiment of 3 independent experiments are shown.