Dose-dependent OPN-induced DC activation
. | HLA-DR . | CD40 . | CD54 . |
|---|---|---|---|
| 0 h | 154.3 | 68.2 | 86.8 |
| Unstimulated | 217.1 | 84.0 | 112.5 |
| OPN, 0.05 μg | 224.0 | 151.8 | 183.1 |
| OPN, 0.5 μg | 243.2 | 280.4 | 244.7 |
. | HLA-DR . | CD40 . | CD54 . |
|---|---|---|---|
| 0 h | 154.3 | 68.2 | 86.8 |
| Unstimulated | 217.1 | 84.0 | 112.5 |
| OPN, 0.05 μg | 224.0 | 151.8 | 183.1 |
| OPN, 0.5 μg | 243.2 | 280.4 | 244.7 |
CD14+ derived DCs were stimulated on day 5 with the indicated concentrations of OPN. The expression of HLA-DR, CD40, or isotype control was analyzed by flow cytometry after 24 hours. Data indicate the corrected mean fluorescence intensity, after subtraction of the fluorescence of the isotype control. Results from one of 2 dose-finding experiments are shown.