Myeloid expansion in spleens of Runx1-excised mice
Genotype . | No. mice . | Time after pIpC, d . | Gr1+ splenocytes, mean ± SD, %*(P) . | Gr1+Mac1+ splenocytes, mean ± SD, %*(P) . | Mac1+ splenocytes, mean ± SD, %*(P) . | Ter119+/CD45- splenocytes, mean ± SD, %*(P)† . |
|---|---|---|---|---|---|---|
| Runx1F/F‡ | 4 | 186-190 | 2.9 ± 0.6 (.999∥¶) | 1.6 ± 0.4 (.008∥) | 6.1 ± 1.4 (.014∥) | 2.9 ± 1.6 (.023∥) |
| Runx1F/F—Tg(Mx1-Cre)‡ | 7 | 186-190 | 2.7 ± 1.9 | 10.2 ± 4.7 | 14.9 ± 8.8 | 20.6 ± 15.5 |
| Runx1F/+ | 4 | 154 | 5.7 ± 1.5 (.020#) | 2.5 ± 0.2 (.020#) | 1.8 ± 0.2 (.020#) | 2.0 ± 1.3 (.136¶#) |
| Runx1F/+—Tg(Mx1-Cre) and Runx1F/rd | 5 | 154 | 7.5 ± 1.2 (.465¶**) | 5.4 ± 2.8 (.029**) | 12.0 ± 0.6 (.006**) | 3.1 ± 2.1 (.715¶**) |
| Runx1F/rd—Tg(Mx1-Cre) | 6 | 154 | 8.3 ± 1.2 | 12.7 ± 5.3 | 4.0 ± 0.5 | 3.8 ± 2.0 |
| Runx1F/F (recipient)‡ | 2 | 96§ | 3.1 ± 1.2 (.098¶††) | 2.5 ± 0.1 (.040††) | 1.9 ± 0.5 (.061¶††) | 2.8 ± 1.7 (.003††) |
| Runx1F/F—Tg(Mx1-Cre) (recipient)‡ | 4 | 96§ | 5.8 ± 1.6 | 7.4 ± 2.2 | 3.9 ± 1.0 | 50.0 ± 9.3 |
Genotype . | No. mice . | Time after pIpC, d . | Gr1+ splenocytes, mean ± SD, %*(P) . | Gr1+Mac1+ splenocytes, mean ± SD, %*(P) . | Mac1+ splenocytes, mean ± SD, %*(P) . | Ter119+/CD45- splenocytes, mean ± SD, %*(P)† . |
|---|---|---|---|---|---|---|
| Runx1F/F‡ | 4 | 186-190 | 2.9 ± 0.6 (.999∥¶) | 1.6 ± 0.4 (.008∥) | 6.1 ± 1.4 (.014∥) | 2.9 ± 1.6 (.023∥) |
| Runx1F/F—Tg(Mx1-Cre)‡ | 7 | 186-190 | 2.7 ± 1.9 | 10.2 ± 4.7 | 14.9 ± 8.8 | 20.6 ± 15.5 |
| Runx1F/+ | 4 | 154 | 5.7 ± 1.5 (.020#) | 2.5 ± 0.2 (.020#) | 1.8 ± 0.2 (.020#) | 2.0 ± 1.3 (.136¶#) |
| Runx1F/+—Tg(Mx1-Cre) and Runx1F/rd | 5 | 154 | 7.5 ± 1.2 (.465¶**) | 5.4 ± 2.8 (.029**) | 12.0 ± 0.6 (.006**) | 3.1 ± 2.1 (.715¶**) |
| Runx1F/rd—Tg(Mx1-Cre) | 6 | 154 | 8.3 ± 1.2 | 12.7 ± 5.3 | 4.0 ± 0.5 | 3.8 ± 2.0 |
| Runx1F/F (recipient)‡ | 2 | 96§ | 3.1 ± 1.2 (.098¶††) | 2.5 ± 0.1 (.040††) | 1.9 ± 0.5 (.061¶††) | 2.8 ± 1.7 (.003††) |
| Runx1F/F—Tg(Mx1-Cre) (recipient)‡ | 4 | 96§ | 5.8 ± 1.6 | 7.4 ± 2.2 | 3.9 ± 1.0 | 50.0 ± 9.3 |
Numbers indicate mean percentage Mac1+, Gr1+, or Mac1+Gr1+ of total splenocytes. Primary pIpC-treated splenocytes were stained with CD45.2-FITC, Mac1-PE, 7AAD-PerCP-Cy5.5, and Gr1-APC and were gated by scatter and 7AAD negativity. Splenocytes from animals that underwent transplantation were stained with CD45.2-FITC, Mac1-PE, CD45.2-PerCP-Cy5.5, and Gr1-APC and were scatter gated.
Numbers indicate mean percentage CD45-Ter119+ of total splenocytes. Splenocytes were stained with CD45.2-FITC, c-Kit-PE, 7AAD-PerCP-Cy5.5, and Ter119-APC. Splenocytes were gated by scatter and 7AAD negativity.
Representative data in Figure 6.
Days after transplantation.
Mann-Whitney U test for comparison of primary Runx1F/F—Tg(Mx1-Cre) to primary Runx1F/F animals.
Not statistically significant.
Mann-Whitney U test for comparison of primary Runx1F/rd—Tg(Mx1-Cre) to primary Runx1F/+ animals.
Mann-Whitney U test for comparison of primary Runx1F/rd—Tg(Mx1-Cre) to primary Runx1F/+—Tg(Mx1-Cre) and Runx1F/rd animals.
Unpaired t test for comparison of mice that underwent transplantation with Runx1F/F—Tg(Mx1-Cre) to mice that underwent transplantation with Runx1F/F animals.