Hematopoietic effects of Runx1 excision are transplantable
. | Donor cell genotype* . | . | |
|---|---|---|---|
| PB counts† . | Runx1F/F, mean ± SD . | Runx1F/F-Tg(Mx1-Cre), mean ± SD . | |
| WBCs, ×103/μL | 6.94 ± 2.3 | 3.55 ± 0.4 | |
| RBCs, ×106/μL | 7.53 ± 1.7 | 9.46 ± 0.5 | |
| Platelets, ×103/μL | 1808 ± 200 | 364 ± 140 | |
| Neutrophils, % | 32.9 ± 4.6 | 40.6 ± 2.6 | |
| Lymphocytes, % | 60.0 ± 4.6 | 49.0 ± 3.1 | |
| Lineage and origin of PB cells‡ | |||
| WBC donor derived, CD45.2+, %§ | 98.7 ± 0.5 | 87.9 ± 3.4 | |
| PB T cells | |||
| CD3+ %∥ | 3.6 ± 1.8 | 9.0 ± 1.7 | |
| CD3+ donor derived, %¶ | 70.9 ± 22.0 | 7.0 ± 4.5 | |
| PB B cells | |||
| B220+, %∥ | 35.4 ± 7.3 | 0.6 ± 0.3 | |
| B220+ donor derived, %¶ | 100.0 ± 0.0 | 97.1 ± 4.5 | |
| PB myeloid cells | |||
| Mac1+, %∥ | 6.4 ± 2.1 | 17.1 ± 3.7 | |
| Mac1+ donor derived, %¶ | 99.6 ± 0.2 | 99.1 ± 0.4 | |
| Gr1+/Mac1+, %∥ | 40.5 ± 71.2 | 67.8 ± 32.9 | |
| Gr1+/Mac1+ donor derived, %¶ | 99.8 ± 0.3 | 98.1 ± 0.8 | |
| Gr1+, %∥ | 1.0 ± 0.4 | 9.8 ± 1.3 | |
| Gr1+ donor derived, %¶ | 45.5 ± 34.7 | 4.9 ± 1.7 | |
. | Donor cell genotype* . | . | |
|---|---|---|---|
| PB counts† . | Runx1F/F, mean ± SD . | Runx1F/F-Tg(Mx1-Cre), mean ± SD . | |
| WBCs, ×103/μL | 6.94 ± 2.3 | 3.55 ± 0.4 | |
| RBCs, ×106/μL | 7.53 ± 1.7 | 9.46 ± 0.5 | |
| Platelets, ×103/μL | 1808 ± 200 | 364 ± 140 | |
| Neutrophils, % | 32.9 ± 4.6 | 40.6 ± 2.6 | |
| Lymphocytes, % | 60.0 ± 4.6 | 49.0 ± 3.1 | |
| Lineage and origin of PB cells‡ | |||
| WBC donor derived, CD45.2+, %§ | 98.7 ± 0.5 | 87.9 ± 3.4 | |
| PB T cells | |||
| CD3+ %∥ | 3.6 ± 1.8 | 9.0 ± 1.7 | |
| CD3+ donor derived, %¶ | 70.9 ± 22.0 | 7.0 ± 4.5 | |
| PB B cells | |||
| B220+, %∥ | 35.4 ± 7.3 | 0.6 ± 0.3 | |
| B220+ donor derived, %¶ | 100.0 ± 0.0 | 97.1 ± 4.5 | |
| PB myeloid cells | |||
| Mac1+, %∥ | 6.4 ± 2.1 | 17.1 ± 3.7 | |
| Mac1+ donor derived, %¶ | 99.6 ± 0.2 | 99.1 ± 0.4 | |
| Gr1+/Mac1+, %∥ | 40.5 ± 71.2 | 67.8 ± 32.9 | |
| Gr1+/Mac1+ donor derived, %¶ | 99.8 ± 0.3 | 98.1 ± 0.8 | |
| Gr1+, %∥ | 1.0 ± 0.4 | 9.8 ± 1.3 | |
| Gr1+ donor derived, %¶ | 45.5 ± 34.7 | 4.9 ± 1.7 | |
Results are for 2 Runx1F/F mice (2 others were lost to fighting) and 4 Runx1F/F-Tg(Mx1-Cre) mice.
Genotypes of donor cells (CD45.2+) transplanted into lethally irradiated B6.SJL (CD45.1+) recipient mice. Mice were killed 14 weeks after transplantation.
Results from automated blood counts.
Cells gated by scatter, CD45.1, and CD45.2.
Indicated is the percentage of total WBCs (pan-CD45+) that were donor derived (CD45.1- and CD45.2+).
Indicated is the percentage of total WBCs (pan-CD45+) that were CD3, B220, Mac-1, or Gr-1 positive.
Indicated is the percentage of total CD3-, B220-, Mac-1-, Mac-1/Gr-1-, or Gr-1-positive WBCs (pan-CD45+) that were donor derived (CD45.2+).