Table 4. CEP-701 inhibits engraftment of... | American Society of Hematology

Table 4.

CEP-701 inhibits engraftment of FLT3/ITD leukemia

Leukemia sample 1, % CD45, sorted			Leukemia sample 9, %CD45
Leukemia sample 1, % CD45, sorted				Sorted		Unsorted
Vehicle	CEP-701	Vehicle	CEP-701	Vehicle	CEP-701
3.3	0	96.1	8.7	98.3	50.4
3.2	0	89.0	74.6	94.2	19.1
4.7	0	95.5	13.2	92.6	76.9
4.6	0	95.6	91.2	75.7	11.7
11.3	0	95.0	86.9	98.0	46.5
19.4	0	90.2	80.0	90.0	—
9.3	0	94.7	1.3	—	—
—	0	95.3	78.2	—	—
8.0^*	0^*	93.9^*	54.2^*	91.5^*	40.9^*

Leukemia sample 1, % CD45, sorted			Leukemia sample 9, %CD45
Leukemia sample 1, % CD45, sorted				Sorted		Unsorted
Vehicle	CEP-701	Vehicle	CEP-701	Vehicle	CEP-701
3.3	0	96.1	8.7	98.3	50.4
3.2	0	89.0	74.6	94.2	19.1
4.7	0	95.5	13.2	92.6	76.9
4.6	0	95.6	91.2	75.7	11.7
11.3	0	95.0	86.9	98.0	46.5
19.4	0	90.2	80.0	90.0	—
9.3	0	94.7	1.3	—	—
—	0	95.3	78.2	—	—
8.0^*	0^*	93.9^*	54.2^*	91.5^*	40.9^*

CD34⁺/CD38⁻ cells (5 × 10³) obtained from leukemia sample 1 (Table 1) were injected via tail vein into 2 groups of 10 sublethally irradiated NOD-SCID mice. In a subsequent experiment, 5 × 10⁶ unsorted cells and 5 × 10³ CD34⁺/CD38⁻ cells from leukemia sample 9 were likewise injected into 4 groups of 8 mice. Mice were then treated with CEP-701 (20 mg/kg) or vehicle twice daily for 5 days every 4 weeks. Some of the mice in the different groups died of apparent infection in the first month of the experiment; thus, final numbers range from 5 to 8 for each group. At sacrifice, the bone marrow cells were analyzed by flow cytometry for human CD45 staining. The % CD45 refers to the percentage of the total marrow that stained for human CD45 (as compared with murine CD45).

—indicates the animal died.

Indicates the mean value for the mice in that group.

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