Polarization of the MTOC in normal uninfected CD4+ PMBCs upon cross-linking with antibody to cell surface molecules
Antibody-coated bead . | No. experiments . | Odds ratio of MTOC polarization in antibody vs isotype control-stimulated cells (% polarization) . | χ2 (DF) . | P . |
|---|---|---|---|---|
| Anti-CD3 (TCR) | 4 | 5.80 (60) | 207 (8) | < .001 |
| Anti-CD54 (ICAM-1) | 7 | 1.21 (27) | 53.2 (14) | < .001 |
| Anti-CD11a (LFA-1) | 5 | 7.80 (64) | 61.0 (10) | < .001 |
| Anti-CD58 (LFA-3) | 3 | 1.76 (26) | 23.0 (6) | < .001 |
| Anti-CD28 | 3 | 3.27 (41) | 60.0 (6) | < .001 |
| Anti-CD18 (LFA-1) | 3 | 10.86 (67) | 168 (6) | < .001 |
| Anti-CD2 | 6 | 5.39 (56) | 175 (12) | < .001 |
| Anti-CD50 (ICAM-3) | 3 | 6.92 (60) | 138 (6) | < .001 |
| Anti-CD25 (IL-2Rα) | 3 | 1.31 (25) | 18.3 (6) | .006 |
Antibody-coated bead . | No. experiments . | Odds ratio of MTOC polarization in antibody vs isotype control-stimulated cells (% polarization) . | χ2 (DF) . | P . |
|---|---|---|---|---|
| Anti-CD3 (TCR) | 4 | 5.80 (60) | 207 (8) | < .001 |
| Anti-CD54 (ICAM-1) | 7 | 1.21 (27) | 53.2 (14) | < .001 |
| Anti-CD11a (LFA-1) | 5 | 7.80 (64) | 61.0 (10) | < .001 |
| Anti-CD58 (LFA-3) | 3 | 1.76 (26) | 23.0 (6) | < .001 |
| Anti-CD28 | 3 | 3.27 (41) | 60.0 (6) | < .001 |
| Anti-CD18 (LFA-1) | 3 | 10.86 (67) | 168 (6) | < .001 |
| Anti-CD2 | 6 | 5.39 (56) | 175 (12) | < .001 |
| Anti-CD50 (ICAM-3) | 3 | 6.92 (60) | 138 (6) | < .001 |
| Anti-CD25 (IL-2Rα) | 3 | 1.31 (25) | 18.3 (6) | .006 |
Latex beads were coated with antibody of the respective specificity and mixed with CD4+ PBMCs from HTLV-1 seronegative control subjects. Odds ratios were calculated by comparing the frequency of polarization of the MTOC toward beads coated with antibodies to each cell surface molecule with the frequency of polarization toward beads coated with isotype control antibodies. The summary odds ratios are reported (weighted mean odds ratio over the indicated number of experiments). DF indicates degrees of freedom.