Table 1.

Hematologic effects of E2 in iron-deficient rats

Vehicle
E2, 0.1 mg/kg
E2, 1 mg/kg
RBC, 104/mm3    
    Basal Diet   589.1 ± 60.5   514.5 ± 65.0*  513.3 ± 65.5* 
    Low-Iron Diet   955.6 ± 89.9†  813.9 ± 70.5*†  680.9 ± 49.5*† 
Hematocrit, %    
    Basal Diet   46.9 ± 1.9   44.1 ± 1.6*  40.8 ± 1.3* 
    Low-Iron Diet   46.0 ± 1.9   46.0 ± 2.0   42.0 ± 2.1* 
Hemoglobin, g/dL    
    Basal Diet   14.9 ± 0.6   14.1 ± 0.5*  13.1 ± 0.6* 
    Low-Iron Diet   13.6 ± 0.9†  14.0 ± 0.8   12.9 ± 0.5* 
MCV, μm3    
    Basal Diet   80.2 ± 7.3   86.8 ± 10.0   80.5 ± 10.2  
    Low-Iron Diet   48.6 ± 5.4†  57.0 ± 6.3*†  61.9 ± 4.0*† 
MCH, pg    
    Basal Diet   25.5 ± 2.5   27.7 ± 2.9   25.9 ± 3.1  
    Low-Iron Diet   14.4 ± 1.9†  17.3 ± 2.2*†  19.0 ± 1.6*† 
MCHC, %    
    Basal Diet   31.8 ± 0.6   31.9 ± 0.6   32.2 ± 0.8  
    Low-Iron Diet   29.6 ± 1.3†  30.4 ± 1.1†  30.7 ± 1.5† 
Plasma iron, μg/dL    
    Basal Diet   322.3 ± 37.2   348.9 ± 71.3   423.8 ± 63.8* 
    Low-Iron Diet   54.0 ± 10.9†  83.2 ± 51.7†  198.6 ± 64.1*† 
TIBC, μg/dL    
    Basal Diet   527.3 ± 28.9   577.4 ± 42.0*  667.8 ± 43.1* 
    Low-Iron Diet   718.7 ± 31.0†  751.8 ± 33.0†  757.0 ± 50.1*† 
Ferritin, ng/mL    
    Basal Diet   97.6 ± 43.7   96.2 ± 39.1   82.1 ± 22.9  
    Low-Iron Diet   16.4 ± 8.2†  17.5 ± 20.1†  36.3 ± 29.1† 
Plasma Epo, mIU/mL    
    Basal Diet   15.0 ± 2.5   19.3 ± 4.8   21.2 ± 5.1  
    Low-Iron Diet
 		 45.5 ± 13.9†
 		 51.3 ± 17.1†
 		 19.7 ± 7.0*
 
Vehicle
E2, 0.1 mg/kg
E2, 1 mg/kg
RBC, 104/mm3    
    Basal Diet   589.1 ± 60.5   514.5 ± 65.0*  513.3 ± 65.5* 
    Low-Iron Diet   955.6 ± 89.9†  813.9 ± 70.5*†  680.9 ± 49.5*† 
Hematocrit, %    
    Basal Diet   46.9 ± 1.9   44.1 ± 1.6*  40.8 ± 1.3* 
    Low-Iron Diet   46.0 ± 1.9   46.0 ± 2.0   42.0 ± 2.1* 
Hemoglobin, g/dL    
    Basal Diet   14.9 ± 0.6   14.1 ± 0.5*  13.1 ± 0.6* 
    Low-Iron Diet   13.6 ± 0.9†  14.0 ± 0.8   12.9 ± 0.5* 
MCV, μm3    
    Basal Diet   80.2 ± 7.3   86.8 ± 10.0   80.5 ± 10.2  
    Low-Iron Diet   48.6 ± 5.4†  57.0 ± 6.3*†  61.9 ± 4.0*† 
MCH, pg    
    Basal Diet   25.5 ± 2.5   27.7 ± 2.9   25.9 ± 3.1  
    Low-Iron Diet   14.4 ± 1.9†  17.3 ± 2.2*†  19.0 ± 1.6*† 
MCHC, %    
    Basal Diet   31.8 ± 0.6   31.9 ± 0.6   32.2 ± 0.8  
    Low-Iron Diet   29.6 ± 1.3†  30.4 ± 1.1†  30.7 ± 1.5† 
Plasma iron, μg/dL    
    Basal Diet   322.3 ± 37.2   348.9 ± 71.3   423.8 ± 63.8* 
    Low-Iron Diet   54.0 ± 10.9†  83.2 ± 51.7†  198.6 ± 64.1*† 
TIBC, μg/dL    
    Basal Diet   527.3 ± 28.9   577.4 ± 42.0*  667.8 ± 43.1* 
    Low-Iron Diet   718.7 ± 31.0†  751.8 ± 33.0†  757.0 ± 50.1*† 
Ferritin, ng/mL    
    Basal Diet   97.6 ± 43.7   96.2 ± 39.1   82.1 ± 22.9  
    Low-Iron Diet   16.4 ± 8.2†  17.5 ± 20.1†  36.3 ± 29.1† 
Plasma Epo, mIU/mL    
    Basal Diet   15.0 ± 2.5   19.3 ± 4.8   21.2 ± 5.1  
    Low-Iron Diet
 		 45.5 ± 13.9†
 		 51.3 ± 17.1†
 		 19.7 ± 7.0*
 

The rats were administered E2 at the indicated doses 3 times a week for 2 months and fed the Basal Diet 5755 (n = 8) or the Low-Iron Purified Diet (n = 9) from TestDiet. Data are presented as mean ± SD.

*

P < .05 (compared with vehicle group).

†

P < .05 (compared with Basal Diet group).

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