MSC immunophenotype have been analyzed by flow cytometry in standard conditions (proliferating cells), after 2-hour incubation with 25 ng/mL HB-EGF, and after culture with differentiation (adipogenic, osteogenic, chondrogenic) media. HB-EGF-treated MSCs lose HER-1 surface expression, which is restored a few hours after removing HB-EGF from the medium (data not shown). Results are expressed as intensity of expression (-, negative; ± weak expression; +, 1 log shift from negative control; ++, 2 log shifts from negative control; +++, 3 log shifts from negative control) and derive from 6 different experiments.