RT-PCR DNA fragment analysis
Cell lines . | HAS1Va . | HAS1Vb . | HAS1 . |
|---|---|---|---|
| KMS-12-PE | + | + | + |
| KMS-12-BM | + | + | + |
| RPMI 8226 | + | - | + |
Cell lines . | HAS1Va . | HAS1Vb . | HAS1 . |
|---|---|---|---|
| KMS-12-PE | + | + | + |
| KMS-12-BM | + | + | + |
| RPMI 8226 | + | - | + |
Transcript levels of HAS1 and novel variants were measured in 3 MM cell lines, RPMI 8226, KMS-12-PE, and KMS-12-BM.
Total RNA for RT-PCR and cell lysate for Western blot analysis were obtained from same cell culture for each cell line.