Table 2.

Production of retrovirus particles with FeLV-C and RD114 envelopes by transient transfection of 293T cells


Vector

Envelope plasmid

No. of transfections

Titer more than 1 × 105 infectious particles per mL (%)
MFGs-LacZ   p61EC (FeLV-C)*  29   3 (11.5)  
MFGs-LacZ
 
pRDF (RD114)
 
11
 
6 (54.5)
 

Vector

Envelope plasmid

No. of transfections

Titer more than 1 × 105 infectious particles per mL (%)
MFGs-LacZ   p61EC (FeLV-C)*  29   3 (11.5)  
MFGs-LacZ
 
pRDF (RD114)
 
11
 
6 (54.5)
 
*

Contains FeLV-C gag pol and env sequences.

Contains only RD114 env sequence.

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