Table 3.

Clonogenic cell growth by ALDHbr CD34+ and ALDHneg CD34+ cells




ALDHbr CD34+

ALDHneg CD34+

ALDHbr CD34neg

P
Myeloid CFUs/100 initiating cells     
    Mean ± SD   26.8 ± 16.95   9.54 ± 11.8   ND   .06  
    Median
 
24
 
3.75
 
ND
 

 



ALDHbr CD34+

ALDHneg CD34+

ALDHbr CD34neg

P
Myeloid CFUs/100 initiating cells     
    Mean ± SD   26.8 ± 16.95   9.54 ± 11.8   ND   .06  
    Median
 
24
 
3.75
 
ND
 

 

linneg SSClo ALDHbr CD34+, linneg SSClo ALDHneg CD34+, and linneg SSClo ALDHbr CD34neg cells were isolated (Figure 1) and cultured in short-term myeloid clonogenic assays, as described in “Materials and methods” (n = 7). The total numbers of myeloid colonies was normalized per 100 cells that initiated each culture. Statistical comparisons (P) were drawn between the linneg SSClo ALDHbr CD34+ and the linneg SSClo ALDHneg CD34+ cell fractions using paired, 1-tailed nonparametric analyses (Wilcoxon signed rank test).

ND indicates none detected.

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