Clonogenic cell growth by ALDHbr CD34+ and ALDHneg CD34+ cells
. | ALDHbr CD34+ . | ALDHneg CD34+ . | ALDHbr CD34neg . | P . |
---|---|---|---|---|
Myeloid CFUs/100 initiating cells | ||||
Mean ± SD | 26.8 ± 16.95 | 9.54 ± 11.8 | ND | .06 |
Median | 24 | 3.75 | ND |
. | ALDHbr CD34+ . | ALDHneg CD34+ . | ALDHbr CD34neg . | P . |
---|---|---|---|---|
Myeloid CFUs/100 initiating cells | ||||
Mean ± SD | 26.8 ± 16.95 | 9.54 ± 11.8 | ND | .06 |
Median | 24 | 3.75 | ND |
linneg SSClo ALDHbr CD34+, linneg SSClo ALDHneg CD34+, and linneg SSClo ALDHbr CD34neg cells were isolated (Figure 1) and cultured in short-term myeloid clonogenic assays, as described in “Materials and methods” (n = 7). The total numbers of myeloid colonies was normalized per 100 cells that initiated each culture. Statistical comparisons (P) were drawn between the linneg SSClo ALDHbr CD34+ and the linneg SSClo ALDHneg CD34+ cell fractions using paired, 1-tailed nonparametric analyses (Wilcoxon signed rank test).
ND indicates none detected.