Enzymatic activity of zebrafish magnesium-dependent neutral sphingomylinase (ZNSMase) in overexpressed COS-7 cells
COS-7 cells . | nSMase activity,*pmol/mg per hour . | Standard deviation, ± . |
|---|---|---|
| Untransfected | 120.2 | 2.3 |
| Mock-transfected | 123.3 | 2.4 |
| pTARGET-ZNSMase | 1321.7 | 4.5 |
COS-7 cells . | nSMase activity,*pmol/mg per hour . | Standard deviation, ± . |
|---|---|---|
| Untransfected | 120.2 | 2.3 |
| Mock-transfected | 123.3 | 2.4 |
| pTARGET-ZNSMase | 1321.7 | 4.5 |
A mammalian-cell transient expression vector with ZNSMase cDNA was transfected into COS-7 cells. nSMase activities were compared between untransfected and transfected cells with mock vector or pTRAGET-ZNSMase at 24 hours after transfection.
Values represent the average of 3 independent determinations.