Newly identified proteins present in immature and mature exosomes
Type . | Name . |
|---|---|
| Chaperones | TCP-1 β,δ,ϵ,θ,ζ |
| cyclophilin A, B | |
| H2-M, la associated invariant chain | |
| Membrane fusion | Annexin 6, 11 |
| Rab 2, 4, 5, 6, 10, 13, 14, 15 | |
| Clathrin, Arp 2/3, Rho A | |
| SNAP 23, syntaxin 7, AP-1 | |
| WD repeat-containing protein 1 | |
| Targeting/adhesion | Thrombospondin 1, Vinculin |
| Plexin A1, C1, Neuropilin 1 | |
| Dectin 1, 2β, 2γ | |
| Integrin β1, CD13, CD166 | |
| Cadherin 1, PGRL, PIRA7 | |
| Cytoskeleton | CAP1, Coronin, advillin, Fascin1, TCTP |
| IQGAP1, Rho GDII, FRL, radixin | |
| Ezrin, Moesin, Talin, Vimentin, myosin heavy chain IX, claudin 1 | |
| dynamin 2, actinin α4, catenin Eα, βcatenin | |
| syntaxin binding protein 3 | |
| Signal transduction | Phospholipase Cα, Fyn, STAT1 |
| Copine 1, c-scr kinase, SHPS-1 | |
| ERK2, Grb 3-3, H-ras | |
| Gi3α, Gsα | |
| Enzymes | Aldehyde reductase, ATP citrate lyase |
| fatty acid synthase, tyk 2, SH2 phosphatase 1 | |
| glucose 6 phosphate isomerase | |
| phosphogluconate deshydrogenase | |
| ATPase Na+/K+ transporting, transketolase | |
| Dipeptidylpeptidase 4, M2 pyruvate kinase | |
| Lipid rafts | Flotillin 1, stomatin, prohibitin |
| Others | Nicastrin, ubiquitine C |
| Ribosomal proteins | |
| MVP, CD98, eEF2 |
Type . | Name . |
|---|---|
| Chaperones | TCP-1 β,δ,ϵ,θ,ζ |
| cyclophilin A, B | |
| H2-M, la associated invariant chain | |
| Membrane fusion | Annexin 6, 11 |
| Rab 2, 4, 5, 6, 10, 13, 14, 15 | |
| Clathrin, Arp 2/3, Rho A | |
| SNAP 23, syntaxin 7, AP-1 | |
| WD repeat-containing protein 1 | |
| Targeting/adhesion | Thrombospondin 1, Vinculin |
| Plexin A1, C1, Neuropilin 1 | |
| Dectin 1, 2β, 2γ | |
| Integrin β1, CD13, CD166 | |
| Cadherin 1, PGRL, PIRA7 | |
| Cytoskeleton | CAP1, Coronin, advillin, Fascin1, TCTP |
| IQGAP1, Rho GDII, FRL, radixin | |
| Ezrin, Moesin, Talin, Vimentin, myosin heavy chain IX, claudin 1 | |
| dynamin 2, actinin α4, catenin Eα, βcatenin | |
| syntaxin binding protein 3 | |
| Signal transduction | Phospholipase Cα, Fyn, STAT1 |
| Copine 1, c-scr kinase, SHPS-1 | |
| ERK2, Grb 3-3, H-ras | |
| Gi3α, Gsα | |
| Enzymes | Aldehyde reductase, ATP citrate lyase |
| fatty acid synthase, tyk 2, SH2 phosphatase 1 | |
| glucose 6 phosphate isomerase | |
| phosphogluconate deshydrogenase | |
| ATPase Na+/K+ transporting, transketolase | |
| Dipeptidylpeptidase 4, M2 pyruvate kinase | |
| Lipid rafts | Flotillin 1, stomatin, prohibitin |
| Others | Nicastrin, ubiquitine C |
| Ribosomal proteins | |
| MVP, CD98, eEF2 |
Forty bands from immature and mature DC exosome preparation separated by SDS-PAGE (shown in Figure 1C) were cut out and analyzed by tandem mass spectrometry. Underlined italicization indicates proteins also identified in exosomes secreted by other cells (see Table S1 for details).