Erythroid progenitor numbers from the BM and spleens of wild-type and Lnk nullizygous mice
Progenitors by tissue type . | Wild type* . | Lnk-/-* . | Fold increase (P)† . | Fold increase per mouse . |
|---|---|---|---|---|
| BM | ||||
| Total cells per mouse, × 106 | 50 ± 12 | 63 ± 10 | 1.3 (.03) | 1.3 |
| BFU-e per 2 × 104 cells | 10 ± 4 | 17 ± 5 | 1.7 (.01) | 2.2 |
| CFU-e per 2 × 105 cells | 593 ± 100 | 517 ± 80 | 0.9 (.04) | 1.1 |
| Spleen | ||||
| Total cells per mouse, × 106 | 150 ± 13 | 286 ± 68 | 1.9 (<.001) | 1.9 |
| BFU-e per 2 × 105 cells | 30 ± 11 | 52 ± 18 | 1.7 (.03) | 3.2 |
| CFU-e per 1 × 106 cells | 230 ± 45 | 546 ± 35 | 2.4 (.01) | 4.6 |
Progenitors by tissue type . | Wild type* . | Lnk-/-* . | Fold increase (P)† . | Fold increase per mouse . |
|---|---|---|---|---|
| BM | ||||
| Total cells per mouse, × 106 | 50 ± 12 | 63 ± 10 | 1.3 (.03) | 1.3 |
| BFU-e per 2 × 104 cells | 10 ± 4 | 17 ± 5 | 1.7 (.01) | 2.2 |
| CFU-e per 2 × 105 cells | 593 ± 100 | 517 ± 80 | 0.9 (.04) | 1.1 |
| Spleen | ||||
| Total cells per mouse, × 106 | 150 ± 13 | 286 ± 68 | 1.9 (<.001) | 1.9 |
| BFU-e per 2 × 105 cells | 30 ± 11 | 52 ± 18 | 1.7 (.03) | 3.2 |
| CFU-e per 1 × 106 cells | 230 ± 45 | 546 ± 35 | 2.4 (.01) | 4.6 |
Mice (8 to 12 weeks old) were used to isolate BM and spleen cells. Total BM and spleen cell numbers (mean ± SD) were enumerated in the presence of 3% acetic acid using a hemocytometer. An equal number of cells was plated in methylcellulose culture and enumerated for 2 days (CFU-e) and 10 days (BFU-e) after plating. The data were obtained from 6 to 8 mice from each genotype. Fold increase = Lnk-/-/wild type. Fold increase per mouse (fifth column) = fold increase (fourth column) × fold increase in total cells.
Data shown are mean plus or minus SD.
P values determined by Student t test.