Effect of paclitaxel and R115777 on cell-cycle progression
Cell-cycle parameters, % . | R115777 . | . | . | ||
|---|---|---|---|---|---|
| . | 0 nM . | 100 nM . | 1000 nM . | ||
| Paclitaxel, 0 nM | |||||
| G0/G1 | 43.10 ± 0.48 | 41.19 ± 2.01 | 33.14 ± 2.74 | ||
| S | 35.62 ± 0.53 | 31.94 ± 1.68 | 36.75 ± 0.64 | ||
| G2/M | 21.27 ± 0.05 | 26.87 ± 0.53 | 30.11 ± 2.18 | ||
| Debris | 9.18 ± 2.30 | 14.25 ± 1.45 | 18.88 ± 0.81 | ||
| Paclitaxel, 1.0 nM | |||||
| G0/G1 | 41.23 ± 4.67 | 24.39 ± 1.63 | 22.48 ± 2.67 | ||
| S | 35.86 ± 3.48 | 34.40 ± 0.57 | 31.26 ± 6.95 | ||
| G2/M | 22.90 ± 2.20 | 41.22 ± 1.67 | 46.26 ± 9.29 | ||
| Debris | 9.58 ± 1.21 | 40.94 ± 0.23 | 59.24 ± 3.04 | ||
| Paclitaxel, 5.0 nM | |||||
| G0/G1 | 25.27 ± 0.88 | 20.01 ± 0.12 | 15.44 ± 4.75 | ||
| S | 44.14 ± 4.44 | 33.98 ± 3.97 | 33.74 ± 10.15 | ||
| G2/M | 30.59 ± 3.65 | 46.01 ± 4.09 | 50.82 ± 6.11 | ||
| Debris | 45.51 ± 4.69 | 68.51 ± 2.56 | 73.91 ± 2.04 | ||
Cell-cycle parameters, % . | R115777 . | . | . | ||
|---|---|---|---|---|---|
| . | 0 nM . | 100 nM . | 1000 nM . | ||
| Paclitaxel, 0 nM | |||||
| G0/G1 | 43.10 ± 0.48 | 41.19 ± 2.01 | 33.14 ± 2.74 | ||
| S | 35.62 ± 0.53 | 31.94 ± 1.68 | 36.75 ± 0.64 | ||
| G2/M | 21.27 ± 0.05 | 26.87 ± 0.53 | 30.11 ± 2.18 | ||
| Debris | 9.18 ± 2.30 | 14.25 ± 1.45 | 18.88 ± 0.81 | ||
| Paclitaxel, 1.0 nM | |||||
| G0/G1 | 41.23 ± 4.67 | 24.39 ± 1.63 | 22.48 ± 2.67 | ||
| S | 35.86 ± 3.48 | 34.40 ± 0.57 | 31.26 ± 6.95 | ||
| G2/M | 22.90 ± 2.20 | 41.22 ± 1.67 | 46.26 ± 9.29 | ||
| Debris | 9.58 ± 1.21 | 40.94 ± 0.23 | 59.24 ± 3.04 | ||
| Paclitaxel, 5.0 nM | |||||
| G0/G1 | 25.27 ± 0.88 | 20.01 ± 0.12 | 15.44 ± 4.75 | ||
| S | 44.14 ± 4.44 | 33.98 ± 3.97 | 33.74 ± 10.15 | ||
| G2/M | 30.59 ± 3.65 | 46.01 ± 4.09 | 50.82 ± 6.11 | ||
| Debris | 45.51 ± 4.69 | 68.51 ± 2.56 | 73.91 ± 2.04 | ||
RPMI8226/S cells were treated for 48 hours before being stained with PI for cell-cycle analysis.