Cytotoxicity of IL-4 cytotoxin to Hodgkin/Reed-Sternberg cells
Cell line . | IC50, ng/mL . | No. experiments performed . | IL-4Rα mRNA expression status . |
|---|---|---|---|
| L428 | 120-200 | 5 | + |
| L1236 | 0.1-0.5 | 8 | ++ |
| L540 | 10-130 | 5 | ++ |
| L591 | 2-20 | 6 | ++ |
| HD-MyZ | 2-35 | 7 | ++ |
| HD-LM2 | More than 1000 | 3 | + |
Cell line . | IC50, ng/mL . | No. experiments performed . | IL-4Rα mRNA expression status . |
|---|---|---|---|
| L428 | 120-200 | 5 | + |
| L1236 | 0.1-0.5 | 8 | ++ |
| L540 | 10-130 | 5 | ++ |
| L591 | 2-20 | 6 | ++ |
| HD-MyZ | 2-35 | 7 | ++ |
| HD-LM2 | More than 1000 | 3 | + |
IC50 is the concentration of IL4 -PE38KDEL at which 50% inhibition of protein synthesis was observed compared with untreated cells.
IL-4Rα mRNA expression was confirmed by RT-PCR analysis, as previously described.29 Band intensity was assigned + for faint band and ++ for strong band.