PCR Primers and probes
PCR product . | Forward primer . | Reverse primer . | Probe . |
|---|---|---|---|
| β-globin | CTCATGGCAAGAAAGTGCTCG | AATTCTTTGCCAAAGTGATGG G | FAMCGTGGATCCTGAGAACTTCAGGCTCCT-TAMRA |
| γ-globin | GGCAACCTGTCCTCTGCCTC | GAAATGGATTGCCAAAACGG | FAMCAAGCTCCTGGGAATGTGCTGGTG-TAMRA |
| ϵ-globin | CAAGCCCGCCTTTGCTAA | TTGCCAAAGTGAGTAGCCAGAA | FAMACTTCAAGCTCCTGGGTAACGTGATGGTGATTAT-TAMRA |
| HS2 core sequence | TTCCAGCATCCTCATCTCTGA | TCACATTCTGTCTCAGGCATC | FAMCAGCCTAGAGTGATGACTCCTATC-TAMRA |
| HS3 core sequence | TATGTATGGGTCAGTGGTCT | CCCTGCTTAGGAGCTTAATC | — |
| HS4 core sequence | AAGGGGTGGACTCCAGAGAC | CTCCTGACTTTCTGTCTAGT | — |
| ϵ-promoter | TTTTAAGTACCATGGAGAACAGG | ATGAAATGACACCATATCAGATAC | FAMGGACACAGGTCAGCCTTGACCA-TAMRA |
| γ-promoter | GAGATTGACAAGAACAGTTTGAC | ATCCAGTGAGGCCAGGGGC | FAMCCTAGTCCAGACGCCATGGGTCAT-TAMRA |
| β-promoter | TGCTTACCAAGCTGTGATTCC | AACGGCAGACTTCTCCTCAGG | FAMAGCCAGTGCCAGAAGAGCCAAGGA-TAMRA |
| Aγ-3′ enhancer MAR 1 | AGAAATGAATCAGCAGAGGCTCA | CATTTTGATCTCACAGTGCTGGTC | — |
| Aγ-3′ enhancer MAR 2 | GGATGGTTCTTAATGAAGGGAAAGT | AAGTGCCATTAGAAATAGTATTCCTCCT | — |
| Aγ-5′ MAR | CCTGGTATCCTCTATGATGGGA | CAAGGCTATTGGTCAAGGCA | — |
| β-5′ MAR | ATAACTAGAAATAGAGGATCCAGTT | CTGAATCCTGCCCCTACCTGGAA | — |
| β-globin IVS MAR | CTCCCTACTTTATTTTCTTTTATTTTTAATTGATAC | CCCTGATTTGGTCAATATGTGTACAC | — |
| ϵ-globin promoter Mϵ-MAR | AACTGTCACCACCTTTAAGGCA | TGCAGATAGATGAGGAGCCAAC | FAMTGCTCCTTTATATGAGGCTTTCTTG-TAMRA |
| HS2-M1 MAR | TGCCTCTACAAAAAGTACAAAAATTAGC | CCCCACAAGAGTCCAAGTAAAAA | FAMATGGTGGTGTGCACCTGTAATCC-TAMRA |
| HS2-M2 MAR | TATTCTTATTTATGATTCAAGCACT | TCATGCTTGGACTATGGGAGGTCA | — |
| HS2-M3 MAR | ACCCCCGCCTTCTGGTT | ACTCAGGTGGTCAGCTTCTCCT | — |
| HS2-M4 MAR | GGATGCCTGAGACAGAATGTGAC | CGGTTCCTGTTCCATATCCTTGTT | — |
| HS2-M5 MAR | AGAAGCGGCAGAATCAGGC | CCCGTATGTGAGCATGTGTCC | — |
PCR product . | Forward primer . | Reverse primer . | Probe . |
|---|---|---|---|
| β-globin | CTCATGGCAAGAAAGTGCTCG | AATTCTTTGCCAAAGTGATGG G | FAMCGTGGATCCTGAGAACTTCAGGCTCCT-TAMRA |
| γ-globin | GGCAACCTGTCCTCTGCCTC | GAAATGGATTGCCAAAACGG | FAMCAAGCTCCTGGGAATGTGCTGGTG-TAMRA |
| ϵ-globin | CAAGCCCGCCTTTGCTAA | TTGCCAAAGTGAGTAGCCAGAA | FAMACTTCAAGCTCCTGGGTAACGTGATGGTGATTAT-TAMRA |
| HS2 core sequence | TTCCAGCATCCTCATCTCTGA | TCACATTCTGTCTCAGGCATC | FAMCAGCCTAGAGTGATGACTCCTATC-TAMRA |
| HS3 core sequence | TATGTATGGGTCAGTGGTCT | CCCTGCTTAGGAGCTTAATC | — |
| HS4 core sequence | AAGGGGTGGACTCCAGAGAC | CTCCTGACTTTCTGTCTAGT | — |
| ϵ-promoter | TTTTAAGTACCATGGAGAACAGG | ATGAAATGACACCATATCAGATAC | FAMGGACACAGGTCAGCCTTGACCA-TAMRA |
| γ-promoter | GAGATTGACAAGAACAGTTTGAC | ATCCAGTGAGGCCAGGGGC | FAMCCTAGTCCAGACGCCATGGGTCAT-TAMRA |
| β-promoter | TGCTTACCAAGCTGTGATTCC | AACGGCAGACTTCTCCTCAGG | FAMAGCCAGTGCCAGAAGAGCCAAGGA-TAMRA |
| Aγ-3′ enhancer MAR 1 | AGAAATGAATCAGCAGAGGCTCA | CATTTTGATCTCACAGTGCTGGTC | — |
| Aγ-3′ enhancer MAR 2 | GGATGGTTCTTAATGAAGGGAAAGT | AAGTGCCATTAGAAATAGTATTCCTCCT | — |
| Aγ-5′ MAR | CCTGGTATCCTCTATGATGGGA | CAAGGCTATTGGTCAAGGCA | — |
| β-5′ MAR | ATAACTAGAAATAGAGGATCCAGTT | CTGAATCCTGCCCCTACCTGGAA | — |
| β-globin IVS MAR | CTCCCTACTTTATTTTCTTTTATTTTTAATTGATAC | CCCTGATTTGGTCAATATGTGTACAC | — |
| ϵ-globin promoter Mϵ-MAR | AACTGTCACCACCTTTAAGGCA | TGCAGATAGATGAGGAGCCAAC | FAMTGCTCCTTTATATGAGGCTTTCTTG-TAMRA |
| HS2-M1 MAR | TGCCTCTACAAAAAGTACAAAAATTAGC | CCCCACAAGAGTCCAAGTAAAAA | FAMATGGTGGTGTGCACCTGTAATCC-TAMRA |
| HS2-M2 MAR | TATTCTTATTTATGATTCAAGCACT | TCATGCTTGGACTATGGGAGGTCA | — |
| HS2-M3 MAR | ACCCCCGCCTTCTGGTT | ACTCAGGTGGTCAGCTTCTCCT | — |
| HS2-M4 MAR | GGATGCCTGAGACAGAATGTGAC | CGGTTCCTGTTCCATATCCTTGTT | — |
| HS2-M5 MAR | AGAAGCGGCAGAATCAGGC | CCCGTATGTGAGCATGTGTCC | — |
—indicates probe not used.