Table 2.

Progenitor cell content of c-MybPlt3/Plt3 and c-MybPlt4/Plt4 bone marrow

Mouse and Stimulus
No. of colonies per 2.5 × 104 bone marrow cells
Blast
G
GM
M
Eo
Meg
Mpl-/- c-MybPlt3/Plt3       
   GM-CSF   0.3 ± 0.6   14 ± 3   15 ± 6   25 ± 6   0   14 ± 8  
   G-CSF   0   10 ± 2   0   0   0   0  
   M-CSF   0   8 ± 2   8 ± 4   54 ± 15   0   0  
   IL-3   12 ± 6   15 ± 3   18 ± 1   16 ± 3   0   64 ± 16  
   EPO   0   0   0   0   0   15 ± 6  
   SCF   8 ± 5   16 ± 6   1 ± 2   0.3 ± 0.6   0   2 ± 2  
   TPO   0   0   0   0   0   0  
   S3E   21 ± 4   11 ± 5   31 ± 9   17 ± 5   0   96 ± 24  
   IL-6   0   0.5 ± 0.9   0   0   0   1 ± 1  
Mpl-/- c-MybPlt4/Plt4       
   GM-CSF   0.5 ± 1.0   24 ± 12   21 ± 14   42 ± 19   0.6 ± 1.3   8 ± 3  
   G-CSF   0   6 ± 1   0   0   0   0  
   M-CSF   0   0.5 ± 1.0   5 ± 0   55 ± 19   0   0  
   IL-3   16 ± 6   45 ± 12   22 ± 11   16 ± 5   0   64 ± 24  
   EPO   0   0   0   0   0   26 ± 17  
   SCF   7 ± 7   20 ± 12   0.6 ± 1.2   0   0   0  
   TPO   0   0   0   0   0   0  
   S3E   17 ± 4   44 ± 15   19 ± 9   14 ± 12   0   113 ± 16  
   IL-6   0   0.6 ± 1.3   0   0   0   0  
Mpl-/- c-Myb+/+       
   GM-CSF   0   15 ± 5   7 ± 4   29 ± 10   2 ± 1   0  
   G-CSF   0   6 ± 3   0   0   0   0  
   M-CSF   0   2 ± 1   2 ± 1   33 ± 14   0   0  
   IL-3   3 ± 2   11 ± 4   9 ± 2   9 ± 4   1 ± 1   5 ± 3  
   EPO   0   0   0   0   0   5 ± 4  
   SCF   2 ± 2   10 ± 1   0.4 ± 0.5   0.6 ± 0.8   0   0  
   TPO   0   0   0   0   0   0  
   S3E   4 ± 3   12 ± 4   9 ± 4   7 ± 3   0.9 ± 0.9   9 ± 6  
   IL-6   0   7 ± 3   0.7 ± 0.5   0   0   0  
Mpl+/+ c-Myb+/+       
   GM-CSF   0   26 ± 9   12 ± 5   31 ± 9   4 ± 2   0  
   G-CSF   0   11 ± 4   0   0   0   0  
   M-CSF   0   1 ± 1   3 ± 2   42 ± 12   0   0  
   IL-3   7 ± 3   21 ± 4   11 ± 4   11 ± 3   3 ± 2   7 ± 1  
   EPO   0   0   0   0   0   7 ± 2  
   SCF   4 ± 2   15 ± 3   0.1 ± 0.1   0.4 ± 0.5   0   0  
   TPO   0   0   0   0   0   3 ± 1  
   S3E   11 ± 4   19 ± 4   12 ± 2   8 ± 5   3 ± 3   21 ± 6  
   IL-6   0   10 ± 3   1.4 ± 0.5   0   0   0.3 ± 0.8  
Mpl+/+ c-MybPlt4/Plt4       
   GM-CSF   0   10 ± 0   15 ± 6   29 ± 5   0.5 ± 0.7   5.5 ± 0.7  
   G-CSF   0   2.0 ± 0   0   0   0   0  
   M-CSF   0   1.5 ± 0.7   3.0 ± 2.8   44 ± 10   0   0  
   IL-3   7.5 ± 2.1   17 ± 6   11 ± 9   9.0 ± 4.2   0   49 ± 14  
   EPO   0   0   0   0   0   19 ± 8  
   SCF   3.0 ± 1.4   7.5 ± 0.7   0.5 ± 0.7   0   0   0  
   TPO   0   0   0   0   0   3.8 ± 2.4  
   S3E   12 ± 6   23 ± 20   27 ± 0.7   14 ± 1.4   0   96 ± 25  
   IL-6
 		 0
 		 0
 		 0
 		 0
 		 0
 		 0
 
Mouse and Stimulus
No. of colonies per 2.5 × 104 bone marrow cells
Blast
G
GM
M
Eo
Meg
Mpl-/- c-MybPlt3/Plt3       
   GM-CSF   0.3 ± 0.6   14 ± 3   15 ± 6   25 ± 6   0   14 ± 8  
   G-CSF   0   10 ± 2   0   0   0   0  
   M-CSF   0   8 ± 2   8 ± 4   54 ± 15   0   0  
   IL-3   12 ± 6   15 ± 3   18 ± 1   16 ± 3   0   64 ± 16  
   EPO   0   0   0   0   0   15 ± 6  
   SCF   8 ± 5   16 ± 6   1 ± 2   0.3 ± 0.6   0   2 ± 2  
   TPO   0   0   0   0   0   0  
   S3E   21 ± 4   11 ± 5   31 ± 9   17 ± 5   0   96 ± 24  
   IL-6   0   0.5 ± 0.9   0   0   0   1 ± 1  
Mpl-/- c-MybPlt4/Plt4       
   GM-CSF   0.5 ± 1.0   24 ± 12   21 ± 14   42 ± 19   0.6 ± 1.3   8 ± 3  
   G-CSF   0   6 ± 1   0   0   0   0  
   M-CSF   0   0.5 ± 1.0   5 ± 0   55 ± 19   0   0  
   IL-3   16 ± 6   45 ± 12   22 ± 11   16 ± 5   0   64 ± 24  
   EPO   0   0   0   0   0   26 ± 17  
   SCF   7 ± 7   20 ± 12   0.6 ± 1.2   0   0   0  
   TPO   0   0   0   0   0   0  
   S3E   17 ± 4   44 ± 15   19 ± 9   14 ± 12   0   113 ± 16  
   IL-6   0   0.6 ± 1.3   0   0   0   0  
Mpl-/- c-Myb+/+       
   GM-CSF   0   15 ± 5   7 ± 4   29 ± 10   2 ± 1   0  
   G-CSF   0   6 ± 3   0   0   0   0  
   M-CSF   0   2 ± 1   2 ± 1   33 ± 14   0   0  
   IL-3   3 ± 2   11 ± 4   9 ± 2   9 ± 4   1 ± 1   5 ± 3  
   EPO   0   0   0   0   0   5 ± 4  
   SCF   2 ± 2   10 ± 1   0.4 ± 0.5   0.6 ± 0.8   0   0  
   TPO   0   0   0   0   0   0  
   S3E   4 ± 3   12 ± 4   9 ± 4   7 ± 3   0.9 ± 0.9   9 ± 6  
   IL-6   0   7 ± 3   0.7 ± 0.5   0   0   0  
Mpl+/+ c-Myb+/+       
   GM-CSF   0   26 ± 9   12 ± 5   31 ± 9   4 ± 2   0  
   G-CSF   0   11 ± 4   0   0   0   0  
   M-CSF   0   1 ± 1   3 ± 2   42 ± 12   0   0  
   IL-3   7 ± 3   21 ± 4   11 ± 4   11 ± 3   3 ± 2   7 ± 1  
   EPO   0   0   0   0   0   7 ± 2  
   SCF   4 ± 2   15 ± 3   0.1 ± 0.1   0.4 ± 0.5   0   0  
   TPO   0   0   0   0   0   3 ± 1  
   S3E   11 ± 4   19 ± 4   12 ± 2   8 ± 5   3 ± 3   21 ± 6  
   IL-6   0   10 ± 3   1.4 ± 0.5   0   0   0.3 ± 0.8  
Mpl+/+ c-MybPlt4/Plt4       
   GM-CSF   0   10 ± 0   15 ± 6   29 ± 5   0.5 ± 0.7   5.5 ± 0.7  
   G-CSF   0   2.0 ± 0   0   0   0   0  
   M-CSF   0   1.5 ± 0.7   3.0 ± 2.8   44 ± 10   0   0  
   IL-3   7.5 ± 2.1   17 ± 6   11 ± 9   9.0 ± 4.2   0   49 ± 14  
   EPO   0   0   0   0   0   19 ± 8  
   SCF   3.0 ± 1.4   7.5 ± 0.7   0.5 ± 0.7   0   0   0  
   TPO   0   0   0   0   0   3.8 ± 2.4  
   S3E   12 ± 6   23 ± 20   27 ± 0.7   14 ± 1.4   0   96 ± 25  
   IL-6
 		 0
 		 0
 		 0
 		 0
 		 0
 		 0
 

Data are the mean ± standard deviation from analysis of 2 to 7 mice of each genotype.

G indicates granulocyte; GM, granulocyte-macrophage; M, macrophage; Eo, eosinophil, Meg, megakaryocyte; S3E, SCF + IL-3 + EPO.

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