Fragment ions detected by ms/ms analysis of RVpTQR
Ions . | Sequences . | T. m/z . | O. m/z . |
|---|---|---|---|
| MH+ | RVpTQR | 739.36 | 739.44 |
| MH+-P | RVTQR | 641.38 | 641.42 |
| MH+-P* | RVTQR* | 624.36 | 624.41 |
| MH+-PΔ | RVTQRΔ | 599.36 | 599.42 |
| MH+-P*Δ | RVTQR*Δ | 582.34 | 582.38 |
| y4-P | VTQR | 485.28 | 485.34 |
| y4-P* | VTQR* | 468.26 | 468.28 |
| y3-P | TQR | 386.22 | 386.25 |
| b3-P | RVT | 339.21 | 339.25 |
| b3-P* | RVT* | 322.19 | 322.23 |
| y2 | QR | 303.18 | 303.19 |
| b2 | RV | 256.18 | 256.22 |
| b2* | RV* | 239.15 | 239.20 |
| a2 | RV | 228.18 | 228.21 |
| a2* | RV* | 211.18 | 211.18 |
| y1 | R | 175.12 | 175.14 |
Ions . | Sequences . | T. m/z . | O. m/z . |
|---|---|---|---|
| MH+ | RVpTQR | 739.36 | 739.44 |
| MH+-P | RVTQR | 641.38 | 641.42 |
| MH+-P* | RVTQR* | 624.36 | 624.41 |
| MH+-PΔ | RVTQRΔ | 599.36 | 599.42 |
| MH+-P*Δ | RVTQR*Δ | 582.34 | 582.38 |
| y4-P | VTQR | 485.28 | 485.34 |
| y4-P* | VTQR* | 468.26 | 468.28 |
| y3-P | TQR | 386.22 | 386.25 |
| b3-P | RVT | 339.21 | 339.25 |
| b3-P* | RVT* | 322.19 | 322.23 |
| y2 | QR | 303.18 | 303.19 |
| b2 | RV | 256.18 | 256.22 |
| b2* | RV* | 239.15 | 239.20 |
| a2 | RV | 228.18 | 228.21 |
| a2* | RV* | 211.18 | 211.18 |
| y1 | R | 175.12 | 175.14 |
The corresponding peptide sequences (Sequences), the theoretical m/z value (T. m/z), and the observed m/z value (O. m/z) of each fragment ion in Figure 1A are shown. The information derived from these fragment ions confirms that Thr133 in this peptide is phosphorylated.
-P indicates peaks resulting from neutral loss of a phosphate group from an ion; *, the loss of an “-NH3”; Δ, the loss of a “-C(NH)2”; and r, immonium ion of arginine.