Differential migration of BMDC/CD11c+ in mice subjected to CLP surgery and challenged with A fumigatus conidia at day 15
CD11c marker and group . | Sham + sal, % gated . | Sham + asp, % gated . | CLP + sal, % gated . | CLP + asp, % gated . |
|---|---|---|---|---|
| BAL | 69 ± 4.9 | 1.9 ± 0.32* | 72.6 ± 1.8 | 0.94 ± 0.5* |
| Lung | 20.0 ± 1.3 | 5.05 ± 0.16* | 10.1 ± 0.8† | 4.4 ± 0.37* |
| LLN | 20.2 ± 1.4 | 22.5 ± 0.75 | 20.3 ± 0.8 | 19.3 ± 3.6 |
| MLN | 11.2 ± 2.3 | 9.4 ± 1.0 | 6.5 ± 1.2 | 7.61 ± 1.3 |
| Spleen | 7.2 ± 1.2 | 8.7 ± 0.45 | 9.9 ± 1.5 | 18.4 ± 2.5*† |
CD11c marker and group . | Sham + sal, % gated . | Sham + asp, % gated . | CLP + sal, % gated . | CLP + asp, % gated . |
|---|---|---|---|---|
| BAL | 69 ± 4.9 | 1.9 ± 0.32* | 72.6 ± 1.8 | 0.94 ± 0.5* |
| Lung | 20.0 ± 1.3 | 5.05 ± 0.16* | 10.1 ± 0.8† | 4.4 ± 0.37* |
| LLN | 20.2 ± 1.4 | 22.5 ± 0.75 | 20.3 ± 0.8 | 19.3 ± 3.6 |
| MLN | 11.2 ± 2.3 | 9.4 ± 1.0 | 6.5 ± 1.2 | 7.61 ± 1.3 |
| Spleen | 7.2 ± 1.2 | 8.7 ± 0.45 | 9.9 ± 1.5 | 18.4 ± 2.5*† |
Mice were subjected to sham or CLP operation and at day 15 they received an intratracheal coinjection of 1 × 106 BMDCs plus 5 × 107 conidia, and 24 hours after they were killed and the tissues (spleen, mesenteric and lung lymph nodes, lung and BAL) were harvested for FACS analysis. Each value is a mean ± SEM of 3 to 4 mice, and the data are representative of 2 experiments.
sal indicates saline; asp, A fumigatus conidia; LLN, lung lymph node; and MLN, mesenteric lymph node.
P < .05 between sham with saline or conidia groups; or CLP with saline or conidia groups
P < .05 between sham and CLP with saline or conidia