Alterations in CD11c+ and MHC II+ DC populations in the lung after sham or CLP surgery
Marker/group . | 1 d, % gated . | 3 d, % gated . | 7 d, % gated . | 15 d, % gated . |
|---|---|---|---|---|
| CD11c | ||||
| Sham | 45.8 ± 2.8 | 30.11 ± 4.32 | 40.6 ± 2.18 | 19.83 ± 1.5 |
| CLP | 32.3 ± 1.4* | 19.6 ± 3.07 | 21.0 ± 3.1* | 28.8 ± 2.1* |
| MHC II | ||||
| Sham | 8.9 ± 2.7 | 5.3 ± 1.13 | 3.9 ± 0.59 | 3.9 ± 1.4 |
| CLP | 15.5 ± 4.8 | 0.7 ± 0.02* | 2.9 ± 1.54 | 1.62 ± 0.2 |
| CD80 | ||||
| Sham | 37.8 ± 5.3 | 21.7 ± 3.6 | 9.3 ± 1.13 | 20.9 ± 8.3 |
| CLP | 35.7 ± 6.7 | 12.8 ± 2.2 | 16.1 ± 1.69* | 17.8 ± 1.3 |
| CD40 | ||||
| Sham | 59.2 ± 5.4 | 42.8 ± 6.7 | 15.2 ± 0.44 | 29.3 ± 3.4 |
| CLP | 46.4 ± 7.1 | 32.7 ± 2.0 | 16.3 ± 1.89 | 32.2 ± 3.5 |
Marker/group . | 1 d, % gated . | 3 d, % gated . | 7 d, % gated . | 15 d, % gated . |
|---|---|---|---|---|
| CD11c | ||||
| Sham | 45.8 ± 2.8 | 30.11 ± 4.32 | 40.6 ± 2.18 | 19.83 ± 1.5 |
| CLP | 32.3 ± 1.4* | 19.6 ± 3.07 | 21.0 ± 3.1* | 28.8 ± 2.1* |
| MHC II | ||||
| Sham | 8.9 ± 2.7 | 5.3 ± 1.13 | 3.9 ± 0.59 | 3.9 ± 1.4 |
| CLP | 15.5 ± 4.8 | 0.7 ± 0.02* | 2.9 ± 1.54 | 1.62 ± 0.2 |
| CD80 | ||||
| Sham | 37.8 ± 5.3 | 21.7 ± 3.6 | 9.3 ± 1.13 | 20.9 ± 8.3 |
| CLP | 35.7 ± 6.7 | 12.8 ± 2.2 | 16.1 ± 1.69* | 17.8 ± 1.3 |
| CD40 | ||||
| Sham | 59.2 ± 5.4 | 42.8 ± 6.7 | 15.2 ± 0.44 | 29.3 ± 3.4 |
| CLP | 46.4 ± 7.1 | 32.7 ± 2.0 | 16.3 ± 1.89 | 32.2 ± 3.5 |
Mice were killed at days 1, 3, 7, and 15 after sham or CLP surgery. Whole-lung samples were collected and processed for fluorescence-activated cell sorting (FACS) analysis. Each value is a mean ± SEM of 3 to 4 mice, and the data are representative of 3 separate experiments.
P < .05 between sham and CLP groups for each time point