Table 1. Published KIT exon 8 AA... | American Society of Hematology

Table 1.

Published KIT exon 8 AA sequences in AML patients

	416	417	418	419	420	421	422
Sources	Leu	Thr	Tyr	Asp	Arg	Leu	Val
Patient no.
1^*	Leu	Thr	Tyr	—	Arg	Leu	Val
2^*	Leu	Thr	Tyr	—	Trp	Gly	Val
3^*	Leu	Thr	Arg	—	Trp	Leu	Val
4^*	Leu	Thr	Ala	—	Arg	Leu	Val
5	Leu	Ile	—	—	Arg	Leu	Val
6	Leu	Thr	Tyr	—	—	Phe	Val
7	Leu	Val	—	—	Arg	Leu	Val
8	Leu	Arg	Gly	—	Arg	Leu	Val
9	Leu	Ile	—	—	Arg	Leu	Val
10	Leu	Asn	—	—	Arg	Leu	Val
11	Leu	Thr	Tyr†	Asp	Gly	Leu	Val
Constructs
Δ419	Leu	Thr	Tyr	—	Arg	Leu	Val
Δ418_419	Leu	Thr	—	—	Arg	Leu	Val
T417I/Δ418_419	Leu	Ile	—	—	Arg	Leu	Val

	416	417	418	419	420	421	422
Sources	Leu	Thr	Tyr	Asp	Arg	Leu	Val
Patient no.
1^*	Leu	Thr	Tyr	—	Arg	Leu	Val
2^*	Leu	Thr	Tyr	—	Trp	Gly	Val
3^*	Leu	Thr	Arg	—	Trp	Leu	Val
4^*	Leu	Thr	Ala	—	Arg	Leu	Val
5	Leu	Ile	—	—	Arg	Leu	Val
6	Leu	Thr	Tyr	—	—	Phe	Val
7	Leu	Val	—	—	Arg	Leu	Val
8	Leu	Arg	Gly	—	Arg	Leu	Val
9	Leu	Ile	—	—	Arg	Leu	Val
10	Leu	Asn	—	—	Arg	Leu	Val
11	Leu	Thr	Tyr†	Asp	Gly	Leu	Val
Constructs
Δ419	Leu	Thr	Tyr	—	Arg	Leu	Val
Δ418_419	Leu	Thr	—	—	Arg	Leu	Val
T417I/Δ418_419	Leu	Ile	—	—	Arg	Leu	Val

Sequences of patients no. 5 to no. 11 were published by Gari et al.⁴ “Sources” indicate the AA sequence of KIT wild type. The lower part of the panel shows the AA sequence of mutations generated by in vitro site-directed mutagenesis using the QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA). The correct sequence of all constructs was confirmed by complete nucleotide sequencing. The shaded areas indicate differences of the AA sequence of patient samples with exon 8 mutations to KIT-WT.

The protein sequence of KIT in exon 8 was reconstructed in 4 patients with AML M4eo that carried exon 8 mutations (patient nos. 1-4).⁹

†

ins Phe Phe.

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