Table 2. Numbers of immunogold particles... | American Society of Hematology

Table 2.

Numbers of immunogold particles for von Willebrand factor in Mks from the spleen of untreated wild-type and GATA-1^low littermates and from mice after 7 days of TPO treatment, as indicated

	von Willebrand factor, no. particles
	Granules	Cytoplasm/DMS
Wild-type mice
Untreated
Light Mk	103 ± 8	91 ± 8
Heavy Mk	-	-
TPO treated
Light Mk	62 ± 10^*	135 ± 4^*
Heavy Mk	33 ± 7^*	138 ± 2^*
GATA-1^low mice
Untreated
Light Mk	21 ± 2	86 ± 2
Heavy Mk	8.0 ± 4.5	81 ± 5
TPO treated
Light Mk	70 ± 10^*	142 ± 11^*
Heavy Mk	63 ± 9^*	183 ± 6^*

	von Willebrand factor, no. particles
	Granules	Cytoplasm/DMS
Wild-type mice
Untreated
Light Mk	103 ± 8	91 ± 8
Heavy Mk	-	-
TPO treated
Light Mk	62 ± 10^*	135 ± 4^*
Heavy Mk	33 ± 7^*	138 ± 2^*
GATA-1^low mice
Untreated
Light Mk	21 ± 2	86 ± 2
Heavy Mk	8.0 ± 4.5	81 ± 5
TPO treated
Light Mk	70 ± 10^*	142 ± 11^*
Heavy Mk	63 ± 9^*	183 ± 6^*

At least 15 to 20 wild-type and more than 50 GATA-1^low Mks were analyzed for the presence of von Willebrand factor-related gold particles. The numbers of gold particles present in 14 μm² of Mks are presented as mean (± SD) of determinations obtained in 5 randomly selected cells for experimental point. The number of gold granules present in 5 randomly selected 14-μm² surfaces outside the cells as control are as follows: for untreated wild-type mice, 2.3 ± 0.5; TPO-treated wild-type mice, 1.5 ± 0.5; GATA-1^low untreated mice, 1.7 ± 0.4; and GATA-1^low TPO-untreated mice, 1.8 ± 0.5.

Data significantly different (P < .05) from those observed in Mks from the corresponding untreated controls

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