TPO and TGF-β1 levels in untreated and TPO-treated (days 7, 14, and 21 after receiving 100 μg TPO/kg of body weight/day for 5 consecutive days, as indicated) wild-type and GATA-1low mice
. | Untreated . | . | Day 7 . | . | Day 14 . | . | Day 21 . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Wild type . | GATA-1low . | Wild type . | GATA-1low . | Wild type . | GATA-1low . | Wild type . | GATA-1low . | ||||
| TPO | ||||||||||||
| Plasma, ng/mL (no. of mice) | 0.76 ± 0.12 (10) | 0.75 ± 0.20 (8) | 6.0 ± 0.5 (10) | 5.7 ± 0.8 (8) | 0.48 ± 0.15 (10) | 0.54 ± 0.16 (8) | 0.58 ± 0.12 (10) | 0.65 ± 0.10 (8) | ||||
| P | - | NS | < .001 | < .001 | NS | NS | NS | NS | ||||
| TGF-1β | ||||||||||||
| Plasma, ng/mL (no. of mice) | 2.1 ± 0.4 (10) | 1.8 ± 0.7 (8) | 16.2 ± 2.7 (10) | 3.5 ± 1.3 (8) | 9.2 ± 2.0 (10) | 2.2 ± 0.8 (8) | 2.0 ± 1.0 (10) | 2.0 ± 1.1 (8) | ||||
| P | - | NS | < .01 | NS | < .01 | NS | NS | NS | ||||
| Extracellular fluids | ||||||||||||
| Bone marrow, ng/mL (no. of mice) | 0.86 ± 0.21 (5) | 1.88 ± 0.33 (4) | ND | ND | 3.45 ± 0.45 (5) | 1.62 ± 0.60 (4) | ND | ND | ||||
| P | - | < .05 | ND | ND | < .01 | NS | ND | ND | ||||
| Spleen, ng/mL (no. of mice) | 1.88 ± 0.39 (5) | 3.20 ± 0.80 (4) | ND | ND | 4.53 ± 1.20 (5) | 3.55 ± 0.65 (4) | ND | ND | ||||
| P | - | < .01 | ND | ND | < .01 | ND | ND | ND | ||||
. | Untreated . | . | Day 7 . | . | Day 14 . | . | Day 21 . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Wild type . | GATA-1low . | Wild type . | GATA-1low . | Wild type . | GATA-1low . | Wild type . | GATA-1low . | ||||
| TPO | ||||||||||||
| Plasma, ng/mL (no. of mice) | 0.76 ± 0.12 (10) | 0.75 ± 0.20 (8) | 6.0 ± 0.5 (10) | 5.7 ± 0.8 (8) | 0.48 ± 0.15 (10) | 0.54 ± 0.16 (8) | 0.58 ± 0.12 (10) | 0.65 ± 0.10 (8) | ||||
| P | - | NS | < .001 | < .001 | NS | NS | NS | NS | ||||
| TGF-1β | ||||||||||||
| Plasma, ng/mL (no. of mice) | 2.1 ± 0.4 (10) | 1.8 ± 0.7 (8) | 16.2 ± 2.7 (10) | 3.5 ± 1.3 (8) | 9.2 ± 2.0 (10) | 2.2 ± 0.8 (8) | 2.0 ± 1.0 (10) | 2.0 ± 1.1 (8) | ||||
| P | - | NS | < .01 | NS | < .01 | NS | NS | NS | ||||
| Extracellular fluids | ||||||||||||
| Bone marrow, ng/mL (no. of mice) | 0.86 ± 0.21 (5) | 1.88 ± 0.33 (4) | ND | ND | 3.45 ± 0.45 (5) | 1.62 ± 0.60 (4) | ND | ND | ||||
| P | - | < .05 | ND | ND | < .01 | NS | ND | ND | ||||
| Spleen, ng/mL (no. of mice) | 1.88 ± 0.39 (5) | 3.20 ± 0.80 (4) | ND | ND | 4.53 ± 1.20 (5) | 3.55 ± 0.65 (4) | ND | ND | ||||
| P | - | < .01 | ND | ND | < .01 | ND | ND | ND | ||||
For statistical analysis, values observed in untreated GATA-1low mice were compared with those observed in untreated wild-type littermates, while values observed in treated animals were compared with those observed in the respective untreated controls.
ND indicates not done; NS, not significantly different (P > .05) from controls; -, not applicable.