Estimated length of cell cycle of individual LTC-ICs
Culture conditions . | Cytokine combination . | Average no. cells*(estimated length of cell cycle, h†) . | . | . | ||
|---|---|---|---|---|---|---|
| . | . | D 3 . | D 5 . | D 7 . | ||
| 3 | SFM | 1.74 (89.8) | 3.81 (62.0) | 5.35 (69.2) | ||
| 4 | SFM3 | 1.92 (76.6) | 5.31 (49.6) | 9.71 (51.1) | ||
| 5 | SFM36 | 2.13 (65.8) | 6.24 (45.3) | 13.46 (44.6) | ||
| 6 | SFM36E | 2.22 (62.4) | 6.17 (45.6) | 14.69 (43.2) | ||
| 7 | SFM36EG | 2.33 (58.8) | 9.18 (37.4) | 31.55 (33.6) | ||
| 8 | SFM36G | 2.79 (48.5) | 9.13 (37.5) | 27.7 (34.9) | ||
| 9 | SFM36EGT | 3.33 (41.3) | 7.46 (41.2) | 15.98 (41.9) | ||
Culture conditions . | Cytokine combination . | Average no. cells*(estimated length of cell cycle, h†) . | . | . | ||
|---|---|---|---|---|---|---|
| . | . | D 3 . | D 5 . | D 7 . | ||
| 3 | SFM | 1.74 (89.8) | 3.81 (62.0) | 5.35 (69.2) | ||
| 4 | SFM3 | 1.92 (76.6) | 5.31 (49.6) | 9.71 (51.1) | ||
| 5 | SFM36 | 2.13 (65.8) | 6.24 (45.3) | 13.46 (44.6) | ||
| 6 | SFM36E | 2.22 (62.4) | 6.17 (45.6) | 14.69 (43.2) | ||
| 7 | SFM36EG | 2.33 (58.8) | 9.18 (37.4) | 31.55 (33.6) | ||
| 8 | SFM36G | 2.79 (48.5) | 9.13 (37.5) | 27.7 (34.9) | ||
| 9 | SFM36EGT | 3.33 (41.3) | 7.46 (41.2) | 15.98 (41.9) | ||
Average number of cells per LTC-IC clone detected on days 3, 5, and 7 was calculated by dividing the sum of cells in each clone counted on that day by the number of clones analyzed. Number of clones analyzed for each cytokine combination varied between 39 and 238. Total number of LTC-ICs analyzed was 699.
The length of cell cycle of individual LTC-IC was calculated by dividing the culture period in hours by log2 of the average number of cells calculated for every time point.