Cytokine combinations and concentrations used to initiate single cell cultures
. | . | Cytokine and cytokine concentration per mL culture medium . | . | . | . | . | . | . | . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Designation . | Combination . | SCF 100 ng . | FL 50 ng . | MGDF 50 ng . | IL-3 100 ng . | IL-6 100 ng . | Epo 3 U . | GM-CSF 20 ng . | TGF-β 10 ng . | |||||||
| 3 | SFM | X | X | X | ||||||||||||
| 4 | SFM3 | X | X | X | X | |||||||||||
| 5 | SFM36 | X | X | X | X | X | ||||||||||
| 6 | SFM36E | X | X | X | X | X | X | |||||||||
| 7 | SFM36EG | X | X | X | X | X | X | X | ||||||||
| 8 | SFM36G | X | X | X | X | X | X | |||||||||
| 9 | SFM36EGT | X | X | X | X | X | X | X | X | |||||||
. | . | Cytokine and cytokine concentration per mL culture medium . | . | . | . | . | . | . | . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Designation . | Combination . | SCF 100 ng . | FL 50 ng . | MGDF 50 ng . | IL-3 100 ng . | IL-6 100 ng . | Epo 3 U . | GM-CSF 20 ng . | TGF-β 10 ng . | |||||||
| 3 | SFM | X | X | X | ||||||||||||
| 4 | SFM3 | X | X | X | X | |||||||||||
| 5 | SFM36 | X | X | X | X | X | ||||||||||
| 6 | SFM36E | X | X | X | X | X | X | |||||||||
| 7 | SFM36EG | X | X | X | X | X | X | X | ||||||||
| 8 | SFM36G | X | X | X | X | X | X | |||||||||
| 9 | SFM36EGT | X | X | X | X | X | X | X | X | |||||||
Designations 3 through 9 are used throughout this report to correspond to the cytokine combinations shown in column 2.
Abbreviations: SCF, stem cell factor; FL, Flt-3 ligand; MGDF, megakaryocyte growth and development factor; IL-3, interleukin-3; IL-6, interleukin-6; Epo, erythropoietin; GM-CSF, granulocyte-macrophage colony-stimulating factor; TGF-β, tumor growth factor-β.