IL-2 bioactivity using human or mouse cells or cell lines
. | Mouse T-cell CTLL-2 ED50, ng/mL . | Mouse NK cells ED50, ng/mL . | Human PBMCs ED50, ng/mL . | Human T-cell Kit-225 ED50, ng/mL . | Intermediate, IL-2R cell TF-1β ED50, ng/mL . |
|---|---|---|---|---|---|
| HuKS-IL-2 | 1.71 | 172.0 | 2.09 | 0.08 | 0.51 |
| Human rIL-2 | 0.65 | 26.0 | 1.51 | 0.07 | 0.71 |
| DI-Leu16-IL-2 | 1.94 | 549.0 | 2.03 | 0.05 | 1.15 |
| Degly-DI-Leu16-IL-2 | 3.30 | NT | 3.42 | 0.09 | 1.99 |
. | Mouse T-cell CTLL-2 ED50, ng/mL . | Mouse NK cells ED50, ng/mL . | Human PBMCs ED50, ng/mL . | Human T-cell Kit-225 ED50, ng/mL . | Intermediate, IL-2R cell TF-1β ED50, ng/mL . |
|---|---|---|---|---|---|
| HuKS-IL-2 | 1.71 | 172.0 | 2.09 | 0.08 | 0.51 |
| Human rIL-2 | 0.65 | 26.0 | 1.51 | 0.07 | 0.71 |
| DI-Leu16-IL-2 | 1.94 | 549.0 | 2.03 | 0.05 | 1.15 |
| Degly-DI-Leu16-IL-2 | 3.30 | NT | 3.42 | 0.09 | 1.99 |
ED50 values are the average of at least 4 determinations except for mouse NK cells (2 assays) and were determined as described in “Materials and methods.”
NT indicates not tested.