Table 1. PCR primers Primer ID .... | American Society of Hematology

Table 1.

PCR primers

Primer ID	Sequence 5′-3′	Location	Annealing temp, °C	Product size, bp
S-2-1	GTTTCTGAGCTGAGATCCTG	Intron 1	58.8	513
AS-2-1	GAGCATCATGGGCCATAGCA	Exon 2	68.7	-
S-2-2	GTCATGTACAACCAGTCTAC	Exon 2	50.6	490
AS-2-2	GCCAAACAATCATTATATACAAG	Intron 2	59.7	-
S-3	TGGATTCTCAGTCGTTCTGGTGA	Intron 2	69.2	308
AS-3	ATGCAGGAGAAGCAACACAC	Intron 3	62.7	-
S-4	TCACTCCTTTCTTCCCCTCTC	Intron 3	64.7	202
AS-4	AATCCCAACCATGAATGCCCTC	Intron 4	72	-
S-5	TCTTCCTGAGGTATGATTATGGTG	Intron 4	57	298
AS-5	AAGAGTTCAGACACAATCTTTTCTC	Intron 5	63.6	-
S-6	GGTCATTGTTATCATGGGACAG	Intron 5	64.1	361
AS-6	TCTTACAATCTAGGCTTCCTTC	Exon 6	60.5	-

Primer ID	Sequence 5′-3′	Location	Annealing temp, °C	Product size, bp
S-2-1	GTTTCTGAGCTGAGATCCTG	Intron 1	58.8	513
AS-2-1	GAGCATCATGGGCCATAGCA	Exon 2	68.7	-
S-2-2	GTCATGTACAACCAGTCTAC	Exon 2	50.6	490
AS-2-2	GCCAAACAATCATTATATACAAG	Intron 2	59.7	-
S-3	TGGATTCTCAGTCGTTCTGGTGA	Intron 2	69.2	308
AS-3	ATGCAGGAGAAGCAACACAC	Intron 3	62.7	-
S-4	TCACTCCTTTCTTCCCCTCTC	Intron 3	64.7	202
AS-4	AATCCCAACCATGAATGCCCTC	Intron 4	72	-
S-5	TCTTCCTGAGGTATGATTATGGTG	Intron 4	57	298
AS-5	AAGAGTTCAGACACAATCTTTTCTC	Intron 5	63.6	-
S-6	GGTCATTGTTATCATGGGACAG	Intron 5	64.1	361
AS-6	TCTTACAATCTAGGCTTCCTTC	Exon 6	60.5	-

- indicates not applicable; bp, base pair.