Colony-forming potential of PCLP1+ or PCLP1- cells from YS
. | CFCs* . | . | . | . | . | . | CFUs-S† . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| E12 YS . | No. of wells . | No. of colonies . | E . | GM . | MK . | Mix . | No. of colonies . | |||||
| PCLP1-Ter119- | 192 | 0 | 0 | 0 | 0 | 0 | 0 | |||||
| PCLP1+Ter119- | 192 | 31 | 23 | 8 | 0 | 0 | 5 | |||||
. | CFCs* . | . | . | . | . | . | CFUs-S† . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| E12 YS . | No. of wells . | No. of colonies . | E . | GM . | MK . | Mix . | No. of colonies . | |||||
| PCLP1-Ter119- | 192 | 0 | 0 | 0 | 0 | 0 | 0 | |||||
| PCLP1+Ter119- | 192 | 31 | 23 | 8 | 0 | 0 | 5 | |||||
The results are expressed as a sum of 2 to 3 separate analyses.
CFCs, colony-forming cells; E, erythroid colonies; GM, granulocyte or macrophage colonies; MK, megakaryocyte colonies; and Mix, mixed colonies.
In vitro methylcellulose CFCs from single cells seeded in 96-well plates. Number of wells analyzed and number of wells with 1 CFC is indicated
In vivo CFUs-S 12 days after injection of 1000 cells in irradiated recipients