Surface phenotype of cultured mast cells after 2 and 4 weeks in vitro
. | 2 wk, % . | . | 4 wk, % . | . | ||
|---|---|---|---|---|---|---|
| Antibody . | AA4-/BGD6+ . | Bone marrow . | AA4-/BGD6+ . | Bone marrow . | ||
| mAb-BGD6 | 98.9 ± 0.7 | 82.6 ± 0.7 | 99.6 ± 0.4 | 99.3 ± 0.6 | ||
| mAb-AA4 | 99.7 ± 0.6 | 87.9 ± 0.4 | 99.5 ± 0.2 | 99.6 ± 0.2 | ||
| c-kit | 99.5 ± 0.2 | 90.3 ± 0.5 | 99.3 ± 0.4 | 98.2 ± 0.5 | ||
| Anti-IgE* | 98.9 ± 0.8 | 85.6 ± 0.2 | 99.7 ± 0.2 | 99.8 ± 0.2 | ||
| Anti-FcϵRI β subunit | 99.1 ± 0.2 | 83.6 ± 0.7 | 99.6 ± 0.3 | 99.1 ± 0.4 | ||
| Anti-CD34 | 57.4 ± 0.6 | 36.8 ± 0.9 | 44.3 ± 0.8 | 38.6 ± 0.7 | ||
. | 2 wk, % . | . | 4 wk, % . | . | ||
|---|---|---|---|---|---|---|
| Antibody . | AA4-/BGD6+ . | Bone marrow . | AA4-/BGD6+ . | Bone marrow . | ||
| mAb-BGD6 | 98.9 ± 0.7 | 82.6 ± 0.7 | 99.6 ± 0.4 | 99.3 ± 0.6 | ||
| mAb-AA4 | 99.7 ± 0.6 | 87.9 ± 0.4 | 99.5 ± 0.2 | 99.6 ± 0.2 | ||
| c-kit | 99.5 ± 0.2 | 90.3 ± 0.5 | 99.3 ± 0.4 | 98.2 ± 0.5 | ||
| Anti-IgE* | 98.9 ± 0.8 | 85.6 ± 0.2 | 99.7 ± 0.2 | 99.8 ± 0.2 | ||
| Anti-FcϵRI β subunit | 99.1 ± 0.2 | 83.6 ± 0.7 | 99.6 ± 0.3 | 99.1 ± 0.4 | ||
| Anti-CD34 | 57.4 ± 0.6 | 36.8 ± 0.9 | 44.3 ± 0.8 | 38.6 ± 0.7 | ||
The data are the X ± SD percent of the total cells that stained with the different antibodies in 7 different experiments. In each experiment at least 100 cells per antibody were counted using fluorescence microscopy in combination with phase contrast microscopy.
Sensitized with 2.5 μg/mL IgE for 24 hours prior to fixation