Comparison of the surface phenotype of freshly isolated AA4-/BGD6+ mast cells and granulated mast cells from mouse bone marrow
Antibody* . | Mast cell precursor,† % . | Granulated mast cell,‡ % . |
|---|---|---|
| mAb-BGD6 | 99.3 ± 0.2 | 99.5 ± 0.1 |
| mAb-AA4 | 0 | 99.3 ± 0.3 |
| Anti-CD34 | 98.2 ± 0.4 | 38.6 ± 0.7 |
| Anti–c-kit | 99.5 ± 0.2 | 99.6 ± 0.3 |
| Anti-CD13 | 99.5 ± 0.3 | 99.4 ± 0.2 |
| Anti-IgE§ | 0 | 99.4 ± 0.2 |
| Anti-FcϵRI β subunit | 0 | 99.4 ± 0.2 |
Antibody* . | Mast cell precursor,† % . | Granulated mast cell,‡ % . |
|---|---|---|
| mAb-BGD6 | 99.3 ± 0.2 | 99.5 ± 0.1 |
| mAb-AA4 | 0 | 99.3 ± 0.3 |
| Anti-CD34 | 98.2 ± 0.4 | 38.6 ± 0.7 |
| Anti–c-kit | 99.5 ± 0.2 | 99.6 ± 0.3 |
| Anti-CD13 | 99.5 ± 0.3 | 99.4 ± 0.2 |
| Anti-IgE§ | 0 | 99.4 ± 0.2 |
| Anti-FcϵRI β subunit | 0 | 99.4 ± 0.2 |
The data are the X ± SD percent of the total cells that stained with the different antibodies in 5 different experiments. In each experiment a minimum of 500 cells per antibody were counted using fluorescence microscopy in combination with phase contrast microscopy.
For mast cell precursors and granulated mast cells, the percentages are 0 for anti-Ly-6G/Ly-6C, anti-CD11b (Mac-1), anti-Thy-1, anti-CD40, anti-CD45R/B220, and anti-Sca-1
Sequentially isolated with mAb-BGD6–conjugated beads after mAb-AA4 beads
Isolated with mAb-AA4–conjugated beads
Sensitized with 2.5 μg/mL IgE for 24 hours prior to fixation