Stages of eosinophil differentiation in CD34+ cells transduced with eGFP or Id2
. | eGFP . | . | Id2 . | . | ||
|---|---|---|---|---|---|---|
. | Day 14 . | Day 17 . | Day 14 . | Day 17 . | ||
| Myeloblast/promyelocyte I | 26.2 ± 14.0 | 16.9 ± 12.0 | 7.9 ± 3.2 | 15.2 ± 2.2 | ||
| Promyelocyte II | 24.7 ± 7.9 | 42.8 ± 7.9 | 20.0 ± 7.3 | 9.1 ± 3.8 | ||
| Myelocyte | 26.6 ± 7.3 | 35.3 ± 11.0 | 61.5 ± 4.2 | 61.9 ± 4.2 | ||
| Metamyelocyte/mature | 3.1 ± 1.7 | 4.4 ± 2.0 | 5.1 ± 1.1 | 9.6 ± 4.8 | ||
. | eGFP . | . | Id2 . | . | ||
|---|---|---|---|---|---|---|
. | Day 14 . | Day 17 . | Day 14 . | Day 17 . | ||
| Myeloblast/promyelocyte I | 26.2 ± 14.0 | 16.9 ± 12.0 | 7.9 ± 3.2 | 15.2 ± 2.2 | ||
| Promyelocyte II | 24.7 ± 7.9 | 42.8 ± 7.9 | 20.0 ± 7.3 | 9.1 ± 3.8 | ||
| Myelocyte | 26.6 ± 7.3 | 35.3 ± 11.0 | 61.5 ± 4.2 | 61.9 ± 4.2 | ||
| Metamyelocyte/mature | 3.1 ± 1.7 | 4.4 ± 2.0 | 5.1 ± 1.1 | 9.6 ± 4.8 | ||
After 14 and 17 days of differentiation, transduced cells were separated from the nontransduced cells, and cytospins were made. Cytospins were stained with May-Grünwald-Giemsa solution. Eosinophil differentiation was expressed as a percentage of myeloblast, promyelocyte I, promyelocyte II, myelocyte, metamyelocyte, and mature eosinophils within the eosinophil lineage. Data are expressed as an average of 3 independent experiments plus or minus SEM.