Stages of neutrophil differentiation in CD34+ cells transduced with eGFP or Id1
. | eGFP . | . | Id1 . | . | ||
|---|---|---|---|---|---|---|
. | Day 14 . | Day 17 . | Day 14 . | Day 17 . | ||
| Myeloblast/promyelocyte I and II | 40.4 ± 19.8 | 34.8 ± 9.5 | 37.3 ± 12.9 | 32.2 ± 14.9 | ||
| Myelocyte | 16.2 ± 3.8 | 27.8 ± 7.8 | 13.7 ± 3.7 | 18.9 ± 4.4 | ||
| Metamyelocyte/banded | 35.1 ± 12.4 | 28.8 ± 0.5 | 40.5 ± 8.1 | 38.8 ± 13.5 | ||
| Segmented | 10.9 ± 3.9 | 10.5 ± 5.0 | 10.0 ± 2.1 | 10.5 ± 1.6 | ||
. | eGFP . | . | Id1 . | . | ||
|---|---|---|---|---|---|---|
. | Day 14 . | Day 17 . | Day 14 . | Day 17 . | ||
| Myeloblast/promyelocyte I and II | 40.4 ± 19.8 | 34.8 ± 9.5 | 37.3 ± 12.9 | 32.2 ± 14.9 | ||
| Myelocyte | 16.2 ± 3.8 | 27.8 ± 7.8 | 13.7 ± 3.7 | 18.9 ± 4.4 | ||
| Metamyelocyte/banded | 35.1 ± 12.4 | 28.8 ± 0.5 | 40.5 ± 8.1 | 38.8 ± 13.5 | ||
| Segmented | 10.9 ± 3.9 | 10.5 ± 5.0 | 10.0 ± 2.1 | 10.5 ± 1.6 | ||
After 14 and 17 days of differentiation, transduced cells were separated from the nontransduced cells, and cytospins were made. Cytospins were stained with May-Grünwald-Giemsa solution. Neutrophil differentiation was expressed as percentage of myeloblast, promyelocyte I, promyelocyte II, myelocyte, and metamyelocyte toward neutrophils with banded and segmented nuclei within the neutrophil lineage. Data are expressed as an average of 3 independent experiments plus or minus SEM.