Stages of eosinophil differentiation in CD34+ cells transduced with eGFP or Id1
. | eGFP . | . | Id1 . | . | ||
|---|---|---|---|---|---|---|
. | Day 14 . | Day 17 . | Day 14 . | Day 17 . | ||
| Myeloblast/promyelocyte I | 17.6 ± 4.8 | 22.8 ± 4.6 | 40.9 ± 4.9 | 53.4 ± 10.3 | ||
| Promyelocyte II | 31.7 ± 6.2 | 21.9 ± 5.7 | 25.4 ± 4.6 | 21.4 ± 6.7 | ||
| Myelocyte | 47.4 ± 5.6 | 51.5 ± 9.9 | 30.2 ± 3.9 | 25.9 ± 10.1 | ||
| Metamyelocyte/mature | 2.9 ± 2.1 | 1.9 ± 1.4 | 2.3 ± 2.2 | 0.1 ± 0.1 | ||
. | eGFP . | . | Id1 . | . | ||
|---|---|---|---|---|---|---|
. | Day 14 . | Day 17 . | Day 14 . | Day 17 . | ||
| Myeloblast/promyelocyte I | 17.6 ± 4.8 | 22.8 ± 4.6 | 40.9 ± 4.9 | 53.4 ± 10.3 | ||
| Promyelocyte II | 31.7 ± 6.2 | 21.9 ± 5.7 | 25.4 ± 4.6 | 21.4 ± 6.7 | ||
| Myelocyte | 47.4 ± 5.6 | 51.5 ± 9.9 | 30.2 ± 3.9 | 25.9 ± 10.1 | ||
| Metamyelocyte/mature | 2.9 ± 2.1 | 1.9 ± 1.4 | 2.3 ± 2.2 | 0.1 ± 0.1 | ||
After 14 and 17 days of differentiation, transduced cells were separated from the nontransduced cells, and cytospins were made. Cytospins were stained with May-Grünwald-Giemsa solution. Eosinophil differentiation was expressed as percentage of myeloblast, promyelocyte I, promyelocyte II, myelocyte, metamyelocyte, and mature eosinophils within the eosinophil lineage. Data are expressed as an average of 7 independent experiments plus or minus the standard error of the mean (SEM).