Table 2.

Amino-terminal sequence analysis of the A2 subunit of thrombin-cleaved factor VIII R372H mutant

Cycle no.
1
2
3
4
5
Residues 373-377   S   V   A   K   K  
A2 subunit of R372H   S   V   A   K   K  
pmol
 		 27.9
 		 10.5
 		 11.9
 		 2.3
 		 3.1
 
Cycle no.
1
2
3
4
5
Residues 373-377   S   V   A   K   K  
A2 subunit of R372H   S   V   A   K   K  
pmol
 		 27.9
 		 10.5
 		 11.9
 		 2.3
 		 3.1
 

The A2 subunit of thrombin-cleaved factor VIII was subjected to 5 cycles of sequencing. Sequences were determined as described in “Materials and methods” and were aligned using the published factor VIII sequences.2  Technical problems with the quantitation of Lys residues render the picomole yield values for this residue unreliable.

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