Specific activity values for factor VIII wild type and R372H
. | Database . | . | . | Recombinant factor VIII . | . | |||
|---|---|---|---|---|---|---|---|---|
| Factor VIII . | Ac, unit/dL . | Ag, unit/dL . | Ac/Ag ratio . | Specific activity, unit/μg . | % of wild type . | |||
| Wild type | NA | NA | NA | 3.53 ± 0.54 | 100 | |||
| R372H | 4.2 ± 1.0 | 122 ± 51 | 0.04 ± 0.03 | 0.024 ± 0.003 | 0.69 | |||
. | Database . | . | . | Recombinant factor VIII . | . | |||
|---|---|---|---|---|---|---|---|---|
| Factor VIII . | Ac, unit/dL . | Ag, unit/dL . | Ac/Ag ratio . | Specific activity, unit/μg . | % of wild type . | |||
| Wild type | NA | NA | NA | 3.53 ± 0.54 | 100 | |||
| R372H | 4.2 ± 1.0 | 122 ± 51 | 0.04 ± 0.03 | 0.024 ± 0.003 | 0.69 | |||
NA, not applicable.
Database values were obtained from the hemophilia A mutation database (HAMSTeRs database; europium.csc.mrc.ac.uk). Ac and Ag refer to factor VIII activity and antigen, respectively. Factor VIII activity was determined by a one-stage clotting assay as described in “Materials and methods.” Factor VIII antigen was determined using ELISA as described in “Materials and methods.” Values represent the mean ± standard deviation from 4 separate determinations.