Comparison of TAP-ANV and a combination of TAP and ANV in TF-initiated plasma clotting, aPTT, and a mouse thrombosis model
. | TF-initiated clotting time,*s . | aPTT,†s . | Occlusion time,‡min . |
|---|---|---|---|
| Control | 42.3 ± 1.3 (n = 7) | 33.2 ± 1.0 (n = 4) | 57 ± 10.2 (n = 5) |
| TAP-ANV | 60.7 ± 1.2 (n = 7)§ | 52.8 ± 2.1 (n = 4)§ | 80 ± 13.5 (n = 6)§ |
| TAP + ANV | 42.5 ± 0.6 (n = 7) | 36.9 ± 0.9 (n = 4)§ | 49 ± 2.4 (n = 5) |
. | TF-initiated clotting time,*s . | aPTT,†s . | Occlusion time,‡min . |
|---|---|---|---|
| Control | 42.3 ± 1.3 (n = 7) | 33.2 ± 1.0 (n = 4) | 57 ± 10.2 (n = 5) |
| TAP-ANV | 60.7 ± 1.2 (n = 7)§ | 52.8 ± 2.1 (n = 4)§ | 80 ± 13.5 (n = 6)§ |
| TAP + ANV | 42.5 ± 0.6 (n = 7) | 36.9 ± 0.9 (n = 4)§ | 49 ± 2.4 (n = 5) |
Values are mean ± SD.
Plasma was supplemented with buffer (control), 2.5 nM fusion protein (TAP-ANV), or 2.5 nM TAP and 2.5 nM ANV (TAP + ANV), and the TF-initiated clotting was determined using a fibrometer
Plasma was supplemented with buffer (control), 50 nM fusion protein (TAP-ANV), or 50 nM TAP and 50 nM ANV (TAP + ANV), and the aPTT was determined using a fibrometer
Mice were injected with PBS (control), 0.23 nmol/kg (10 μg/kg) fusion protein (TAP-ANV), or a mixture of 0.23 nmol/kg TAP and 0.23 nmol/kg ANV (TAP + ANV), and the occlusion time was determined as described in “Materials and methods”
P ≤ .01 versus control (2-tailed t test)