Pooled human plasma (100 μL) was mixed with an equal volume of an inhibitor dissolved in buffer (10 mM Tris, pH 7.4; 0.15 M NaCl; 1 mg/mL BSA; 0.02% NaN₃) at varying concentrations. Dade Innovin was diluted 1:100 with buffer (75 mM NaCl, 12.5 mM CaCl₂, 0.5 mg/mL BSA, 0.02% NaN₃) for the assay. Clotting times were determined with an ACL 200 coagulation analyzer.

Concentration of inhibitor that prolongs the TF-induced clotting time 1.5-fold relative to control (ie, from 40.7 to 61.1 seconds)

Calculated by dividing [Inhibitor]_{1.5 × CT} for TFPI by the value for the other specified inhibitor