Oligonucleotide primer sequences used for CMP-sialic acid transporter amplifications
Species primers . | Sequences 5′ to 3′ . | cDNA positions* . | Comments . |
|---|---|---|---|
| Human | |||
| TRN-1s | GGCTGTCGGGGAACCATGGCTGC | - 15..8 | cDNA cloning |
| NTR-6as | CCGGAACAAGAGAACAGTTAGTACAG | 1407..1382 | cDNA cloning |
| TRN-2s | GAACCATGGCTGCCCCGAGAGAC | - 5..18 | SfaNI digestion |
| TRN-5as | TCCACATAACCGCACTGATCATGCC | 1144..1120 | SfaNI digestion |
| NTR-3s | CTGGATGCAGCAGTGTACCAGGTG | - 334..-357 | Hybridization probe |
| NTR-4as | GGAAAGGACAATGGCCGCTGCTGC | 848..825 | Hybridization probe |
| INT-6s | GTACAGTATGTCATGTGTCACACAACC | intron 5 | Intron 6 amplification |
| NTR-4as | GGAAAGGACAATGGCCGCTGCTGC | 848..825 | Intron 6 amplification |
| Hamster | |||
| HA-s | CTCTGCACCATGGCTCAGGCGAG | - 9..14 | cDNA cloning |
| HA-as | AATCTCACACCAATGACTCTTTCT | 1230..1180 | cDNA cloning |
| HA-Ex6s | CAGGGATCGTGGTGACGTTAGTTGG | 647..671 | Intron 6 amplification |
| HA-NTRas | AGAAAGAACAATGGCTGCAGCTGC | 849..826 | Intron 6 amplification |
Species primers . | Sequences 5′ to 3′ . | cDNA positions* . | Comments . |
|---|---|---|---|
| Human | |||
| TRN-1s | GGCTGTCGGGGAACCATGGCTGC | - 15..8 | cDNA cloning |
| NTR-6as | CCGGAACAAGAGAACAGTTAGTACAG | 1407..1382 | cDNA cloning |
| TRN-2s | GAACCATGGCTGCCCCGAGAGAC | - 5..18 | SfaNI digestion |
| TRN-5as | TCCACATAACCGCACTGATCATGCC | 1144..1120 | SfaNI digestion |
| NTR-3s | CTGGATGCAGCAGTGTACCAGGTG | - 334..-357 | Hybridization probe |
| NTR-4as | GGAAAGGACAATGGCCGCTGCTGC | 848..825 | Hybridization probe |
| INT-6s | GTACAGTATGTCATGTGTCACACAACC | intron 5 | Intron 6 amplification |
| NTR-4as | GGAAAGGACAATGGCCGCTGCTGC | 848..825 | Intron 6 amplification |
| Hamster | |||
| HA-s | CTCTGCACCATGGCTCAGGCGAG | - 9..14 | cDNA cloning |
| HA-as | AATCTCACACCAATGACTCTTTCT | 1230..1180 | cDNA cloning |
| HA-Ex6s | CAGGGATCGTGGTGACGTTAGTTGG | 647..671 | Intron 6 amplification |
| HA-NTRas | AGAAAGAACAATGGCTGCAGCTGC | 849..826 | Intron 6 amplification |
Numbering considers the A of the starting codon ATG as position 1.