Summary of expression of FGFR3 on primary MM cells in relation to sensitivity to CHIR-258
Patient no. . | FGFR3 by flow cytometry . | FGFR3 genotype . | N- and K-Ras genotype . | % annexin V, DMSO . | % annexin V, 500 nM CHIR-258 . | Increase in annexin V, % . |
|---|---|---|---|---|---|---|
| 1 | ND | WT | WT | 9.0 | 21.8 | 20.9 |
| 2 | 1.3 | WT | WT | 10.4 | 8.6 | –1.8 |
| 3 | 11.6 | WT | WT | 9.8 | 42.1 | 32.3 |
| 4 | 2.7 | WT | WT | 6.8 | 25.7 | 18.9 |
| 5 | 26.9 | WT | WT | 10.1 | 24.5 | 14.4 |
| 6 | 1.1 | ND | ND | 8.8 | 10.2 | 1.4 |
| 7 | 0.95 | ND | ND | 15.3 | 16.0 | 0.7 |
| 8 | 0.79 | ND | ND | 20.9 | 20.7 | –0.2 |
| 9 | 1.02 | ND | ND | 12.8 | 13.4 | 0.6 |
| 10 | 0.78 | ND | ND | 15.0 | 17.1 | 2.1 |
Patient no. . | FGFR3 by flow cytometry . | FGFR3 genotype . | N- and K-Ras genotype . | % annexin V, DMSO . | % annexin V, 500 nM CHIR-258 . | Increase in annexin V, % . |
|---|---|---|---|---|---|---|
| 1 | ND | WT | WT | 9.0 | 21.8 | 20.9 |
| 2 | 1.3 | WT | WT | 10.4 | 8.6 | –1.8 |
| 3 | 11.6 | WT | WT | 9.8 | 42.1 | 32.3 |
| 4 | 2.7 | WT | WT | 6.8 | 25.7 | 18.9 |
| 5 | 26.9 | WT | WT | 10.1 | 24.5 | 14.4 |
| 6 | 1.1 | ND | ND | 8.8 | 10.2 | 1.4 |
| 7 | 0.95 | ND | ND | 15.3 | 16.0 | 0.7 |
| 8 | 0.79 | ND | ND | 20.9 | 20.7 | –0.2 |
| 9 | 1.02 | ND | ND | 12.8 | 13.4 | 0.6 |
| 10 | 0.78 | ND | ND | 15.0 | 17.1 | 2.1 |
FGFR3 expression on CD138 primary MM cells was analyzed by flow cytometry and the values indicate fold increase in mean fluorescence over isotype control. CD138-selected cells were screened for the FGFR3 and N- and K-Ras mutations.
ND indicates not determined.