Oligonucleotides used for PCR and EMSA
. | 5′-sequence-3′ . |
|---|---|
| PCR primers | |
| JAK2-f | AAGCCACTGCCAGAAACTTG |
| JAK2-r | ACTGAATTCCACCGTTTCCA |
| β2-M-f | TGTCTTTCAGCAAGGACTGG |
| β2-M-r | GATGCTGCTTACATGTCTCG |
| SOCS-1-f | AGAGCTTCGACTGCCTCTTC |
| SOCS-1-r | AGGGGAAGGAGCTCAGGTAG |
| Cyclin D1-f | CGTGGCCTCTAAGATGAAGG |
| Cyclin D1-r | CTGGCATTTTGGAGAGGAAG |
| RB1-f | GGAAGCAACCCTCCTAAACC |
| RB1-r | TTTCTGCTTTTGCATTCGTG |
| HPRT-1-f | GACCAGTCAACAGGGGACAT |
| HPRT-1-r | CTTGCGACCTTGACCATCTT |
| SOCS-1-mRNAf | CACCATGGTAGCACACAACCAGGTGG |
| SOCS-1-mRNAr | TCAAATCTGGAAGGGGAAGGAGCTC |
| EMSA probe | |
| β-casein-f | AGGAGATTTCTAGGAATTCAATCC |
| β-casein-r | AGGGGATTGAATTCCTAGAAATCT |
. | 5′-sequence-3′ . |
|---|---|
| PCR primers | |
| JAK2-f | AAGCCACTGCCAGAAACTTG |
| JAK2-r | ACTGAATTCCACCGTTTCCA |
| β2-M-f | TGTCTTTCAGCAAGGACTGG |
| β2-M-r | GATGCTGCTTACATGTCTCG |
| SOCS-1-f | AGAGCTTCGACTGCCTCTTC |
| SOCS-1-r | AGGGGAAGGAGCTCAGGTAG |
| Cyclin D1-f | CGTGGCCTCTAAGATGAAGG |
| Cyclin D1-r | CTGGCATTTTGGAGAGGAAG |
| RB1-f | GGAAGCAACCCTCCTAAACC |
| RB1-r | TTTCTGCTTTTGCATTCGTG |
| HPRT-1-f | GACCAGTCAACAGGGGACAT |
| HPRT-1-r | CTTGCGACCTTGACCATCTT |
| SOCS-1-mRNAf | CACCATGGTAGCACACAACCAGGTGG |
| SOCS-1-mRNAr | TCAAATCTGGAAGGGGAAGGAGCTC |
| EMSA probe | |
| β-casein-f | AGGAGATTTCTAGGAATTCAATCC |
| β-casein-r | AGGGGATTGAATTCCTAGAAATCT |
f indicates forward; r, reverse; β2-M, β2-microglobulin.