Enumeration of CD4+/CD25+ cells in pancreatic lymph node
. | No. . | No. cells × 103/pan-LN ± SD . | % CD4+/CD25+ ± SD . |
|---|---|---|---|
| NOD | 3 | 28.9 ± 3.7 | 2.4 ± 0.7 |
| B10.BR | 3 | 26.1 ± 1.4 | 1.6 ± 0.3 |
| anti-CD8, chimeric | 3 | 8.2 ± 6.0 | 1.7 ± 0.8 |
| Nonchimeric anti-CD8 + anti-CD4 | 4 | 58.4 ± 4.8 | 3.3 ± 1.6 |
| Full conditioning, chimeric | 2 | 46.4 ± 2.4 | 1.6 ± 0.1 |
. | No. . | No. cells × 103/pan-LN ± SD . | % CD4+/CD25+ ± SD . |
|---|---|---|---|
| NOD | 3 | 28.9 ± 3.7 | 2.4 ± 0.7 |
| B10.BR | 3 | 26.1 ± 1.4 | 1.6 ± 0.3 |
| anti-CD8, chimeric | 3 | 8.2 ± 6.0 | 1.7 ± 0.8 |
| Nonchimeric anti-CD8 + anti-CD4 | 4 | 58.4 ± 4.8 | 3.3 ± 1.6 |
| Full conditioning, chimeric | 2 | 46.4 ± 2.4 | 1.6 ± 0.1 |
Pancreatic lymph nodes were harvested and digested with collagenase. Single-cell suspensions were then analyzed for expression of CD4 and CD25 by flow cytometry. The percentage of CD4+/CD25+ cells per pan-LN ± SD are represented.