Sequences of primers and probes used for Q-RT-PCR
Gene and type . | Sequence . | Position . | Size, bp . |
|---|---|---|---|
| For both | |||
| RPL19 | |||
| Forward | ATGTATCACAGCCTGTACCTG | 355-375 | |
| Probe | TTTCGTGCTTCCTTGGTCTTAGACCT (AS) | 487-512 | 233 |
| Reverse | TTCTTGGTCTCCTCCTCCTTG | 567-587 | |
| HPRT | |||
| Forward | AGCTTGCTGGTGAAAAGGAC | 571-590 | |
| Probe | GTTGGATACAGGCCAGACTTTGTTG | 601-625 | 189 |
| Reverse | CAACTTGCGCTCATCTTAGG | 740-759 | |
| For pCYTO/CHEM | |||
| TNFα | |||
| Forward | CCCCAAAGGGATGAGAAGTTC | 328-348 | |
| Probe | TCATCAGTTCTATGGCCCAGACCCTCA | 365-391 | 100 |
| Reverse | GCTTGTCACTCGAATTTTGAGAA | 405-427 | |
| IL1β | |||
| Forward | TGAAGTTGACGGACCCCAAA | 137-156 | |
| Probe | CCTTTGACCTGGGCTGTCCAGATGAG | 178-203 | 101 |
| Reverse | TGATGTGCTGCTGTGAGATT | 218-237 | |
| IL6 | |||
| Forward | CTTCAACCAAGAGGTAAAAGATTTA | 487-511 | |
| Probe | CCTTCCTACCCCAATTTCCAATGCTCTCCTA | 523-553 | 99 |
| Reverse | TAGCCACTCCTTCTGTGACTCC | 565-586 | |
| MCP-1 | |||
| Forward | GTTGGCTCAGCCAGATGCA | 151-169 | |
| Probe | TTAACGCCCCACTCACCTGCTGCTACT | 171-197 | 81 |
| Reverse | AGCCTACTCATTGGGATCATCTTG | 208-231 | |
| MIP-2 | |||
| Forward | CAGACAGAAGTCATAGCCACTCTCA | 236-260 | |
| Probe | TTGACCCTGAAGCCCCCCTGGTT | 282-304 | 101 |
| Reverse | CCTTTGTTCAGTATCTTTTGGATGATTT | 309-336 | |
| For pPC/HYPO | |||
| TM* | |||
| Forward | TCCATTGCCAGCCTGTCC | 1728-1745 | |
| Probe | TGGTGGTGGCGCTTTTGGCG | 1747-1766 | 65 |
| Reverse | TGCTTCTTGCGCAGGTGAC | 1774-1792 | |
| EPCR* | |||
| Forward | AAAGGACTTGAGAGCATTTGTG | 993-1014 | |
| Probe | CCCACTTCTCAGACCAGCTACACAAAGCA (AS) | 1013-1041 | 82 |
| Reverse | CCAAGTCTATGGTCTGATTTCC | 1053-1074 | |
| PC* | |||
| Forward | GCACACCAACAACTATGGCATCTA | 1291-1314 | |
| Probe | CCAAAGTGGGAAGCTACCTCAAATGGATTC | 1317-1346 | 101 |
| Reverse | GCTTCTGGCTCTTAAGGGAGACA | 1369-1391 | |
| iNOS | |||
| Forward | GCCACCAACAATGGCAACA | 866-884 | |
| Probe | CCCCCAGCGGAGTGACGGC | 907-925 | 103 |
| Reverse | CGTACCGGATGAGCTGTGAA | 949-968 | |
| HIF1α | |||
| Forward | CAGCTCCTCAGCATTTCACCA | 3024-3044 | |
| Probe | TGCATTGCTGTAGTGTCATTTAAAATGCACCTTT | 3046-3079 | 90 |
| Reverse | GGGACAAACTCCCTCACCAA | 3094-3113 | |
| VEGF | |||
| Forward | GAAAGACCGATTAACCATGTCA | 1023-1044 | |
| Probe | ACCATGCCATCATCGTCACCG | 1047-1067 | 68 |
| Reverse | TGGATTAAGGACTGTTCTGTCAA | 1068-1090 |
Gene and type . | Sequence . | Position . | Size, bp . |
|---|---|---|---|
| For both | |||
| RPL19 | |||
| Forward | ATGTATCACAGCCTGTACCTG | 355-375 | |
| Probe | TTTCGTGCTTCCTTGGTCTTAGACCT (AS) | 487-512 | 233 |
| Reverse | TTCTTGGTCTCCTCCTCCTTG | 567-587 | |
| HPRT | |||
| Forward | AGCTTGCTGGTGAAAAGGAC | 571-590 | |
| Probe | GTTGGATACAGGCCAGACTTTGTTG | 601-625 | 189 |
| Reverse | CAACTTGCGCTCATCTTAGG | 740-759 | |
| For pCYTO/CHEM | |||
| TNFα | |||
| Forward | CCCCAAAGGGATGAGAAGTTC | 328-348 | |
| Probe | TCATCAGTTCTATGGCCCAGACCCTCA | 365-391 | 100 |
| Reverse | GCTTGTCACTCGAATTTTGAGAA | 405-427 | |
| IL1β | |||
| Forward | TGAAGTTGACGGACCCCAAA | 137-156 | |
| Probe | CCTTTGACCTGGGCTGTCCAGATGAG | 178-203 | 101 |
| Reverse | TGATGTGCTGCTGTGAGATT | 218-237 | |
| IL6 | |||
| Forward | CTTCAACCAAGAGGTAAAAGATTTA | 487-511 | |
| Probe | CCTTCCTACCCCAATTTCCAATGCTCTCCTA | 523-553 | 99 |
| Reverse | TAGCCACTCCTTCTGTGACTCC | 565-586 | |
| MCP-1 | |||
| Forward | GTTGGCTCAGCCAGATGCA | 151-169 | |
| Probe | TTAACGCCCCACTCACCTGCTGCTACT | 171-197 | 81 |
| Reverse | AGCCTACTCATTGGGATCATCTTG | 208-231 | |
| MIP-2 | |||
| Forward | CAGACAGAAGTCATAGCCACTCTCA | 236-260 | |
| Probe | TTGACCCTGAAGCCCCCCTGGTT | 282-304 | 101 |
| Reverse | CCTTTGTTCAGTATCTTTTGGATGATTT | 309-336 | |
| For pPC/HYPO | |||
| TM* | |||
| Forward | TCCATTGCCAGCCTGTCC | 1728-1745 | |
| Probe | TGGTGGTGGCGCTTTTGGCG | 1747-1766 | 65 |
| Reverse | TGCTTCTTGCGCAGGTGAC | 1774-1792 | |
| EPCR* | |||
| Forward | AAAGGACTTGAGAGCATTTGTG | 993-1014 | |
| Probe | CCCACTTCTCAGACCAGCTACACAAAGCA (AS) | 1013-1041 | 82 |
| Reverse | CCAAGTCTATGGTCTGATTTCC | 1053-1074 | |
| PC* | |||
| Forward | GCACACCAACAACTATGGCATCTA | 1291-1314 | |
| Probe | CCAAAGTGGGAAGCTACCTCAAATGGATTC | 1317-1346 | 101 |
| Reverse | GCTTCTGGCTCTTAAGGGAGACA | 1369-1391 | |
| iNOS | |||
| Forward | GCCACCAACAATGGCAACA | 866-884 | |
| Probe | CCCCCAGCGGAGTGACGGC | 907-925 | 103 |
| Reverse | CGTACCGGATGAGCTGTGAA | 949-968 | |
| HIF1α | |||
| Forward | CAGCTCCTCAGCATTTCACCA | 3024-3044 | |
| Probe | TGCATTGCTGTAGTGTCATTTAAAATGCACCTTT | 3046-3079 | 90 |
| Reverse | GGGACAAACTCCCTCACCAA | 3094-3113 | |
| VEGF | |||
| Forward | GAAAGACCGATTAACCATGTCA | 1023-1044 | |
| Probe | ACCATGCCATCATCGTCACCG | 1047-1067 | 68 |
| Reverse | TGGATTAAGGACTGTTCTGTCAA | 1068-1090 |
Not used in this study