All insertion site sequences detected in different myeloid cell preparations (CD15 source) were aligned to the human genome using the NCB1BlastN (http://www.nebi.nlm.nih.gov/blast/), the University of California, Santa Cruz, BLAT search tools (http://genome.uese.edu/), and the Ensembl database (http://www.ensembi.org/). The genomic sequence information of these myeloid integration sites that were mappable to the human genome was used to design new primers to enable a specific PCR tracking in lymphoid CD3⁺ cells. The “Tracking” column summarizes whether each insertion site sequence was detectable in purified T cells or not. Details of the tracking analysis are demonstrated in Figures 3C and 4B.

Length denotes the size of the genomic DNA part of the LAM-PCR amplicon without linker and LTR sequence; E value, expectation value; tracking, PCR tracking of specific clones in FACS-purified CD3⁺ cells; CD15, granulocytes; chr, chromosome; neg, no detection; pos, positive detection; CD14, monocytes; LTC-IC, long-term culture-initiating cells; n.d., not done; — indicates for clones 7328 and 3484 that they were too short, and for clone 4381 that it was large enough to be blasted but there was either no match or several hits. Boldfaced, italicized sections of sequences indicate the beginning of the vector long-term terminal repeat (LTR).