Molecular evidence of transposition: genomic annotations of 9 insertions of the FVIII transposon
Transposon IR: Target TA → Flanking sequence . | Map position . | Within gene? . | Integration annotation . |
|---|---|---|---|
| GACTATTTGACGTTCACATTGACCTTTTTAGTTTTCCATG | 6-B1 | Yes | Intron of Sec811 |
| GACTAATCTGCCGCCAAACGCAGACACCATTGCATACACT | 3-E1 | No | 176 kb from Rap2b |
| GACTACCAAAGTTGGGCAGGTCACTGGGCTGCCTTTCCTT | 3-H1 | No | 424 kb from Ttc5 |
| GACTACCAAACCTAGTCTATAGTCAACCACACAGAATTTA | 16-B2 | Yes | Intron of Il1rap |
| GACTACCTAACCCAGCACCCCACTCCCCTAGTCCCGACGG | 4-D2 | No | 1.5 kb from NM144941 |
| GACTATGTACCCGACTTGGATCCAGAGGGTGTATATGTAT | X-A5 | No | 248 kb from novel gene |
| GACTACTGGGAATGATGGACAGTCCCACACTCAGGATCAC | 13-D1 | No | 56 kb from Foxd1 |
| GACTATTTAGAAAATGTAGGCATTTACAAGAATATCCAAA | 7-B5 | No | 156kb from 008905 |
| GACTATATATTTAGAGAGCTTTAGAAAGTTACTGAAGCTT | 1-H1 | Yes | Intron of NM013862 |
Transposon IR: Target TA → Flanking sequence . | Map position . | Within gene? . | Integration annotation . |
|---|---|---|---|
| GACTATTTGACGTTCACATTGACCTTTTTAGTTTTCCATG | 6-B1 | Yes | Intron of Sec811 |
| GACTAATCTGCCGCCAAACGCAGACACCATTGCATACACT | 3-E1 | No | 176 kb from Rap2b |
| GACTACCAAAGTTGGGCAGGTCACTGGGCTGCCTTTCCTT | 3-H1 | No | 424 kb from Ttc5 |
| GACTACCAAACCTAGTCTATAGTCAACCACACAGAATTTA | 16-B2 | Yes | Intron of Il1rap |
| GACTACCTAACCCAGCACCCCACTCCCCTAGTCCCGACGG | 4-D2 | No | 1.5 kb from NM144941 |
| GACTATGTACCCGACTTGGATCCAGAGGGTGTATATGTAT | X-A5 | No | 248 kb from novel gene |
| GACTACTGGGAATGATGGACAGTCCCACACTCAGGATCAC | 13-D1 | No | 56 kb from Foxd1 |
| GACTATTTAGAAAATGTAGGCATTTACAAGAATATCCAAA | 7-B5 | No | 156kb from 008905 |
| GACTATATATTTAGAGAGCTTTAGAAAGTTACTGAAGCTT | 1-H1 | Yes | Intron of NM013862 |
Nine insertions of the hFVIII transposon were cloned by linker-mediated PCR. Liver genomic DNA from the animals in Figure 4B was used as template for splinkerette PCR reactions. Recovered flanking sequences were used to search the ENSMBL database to determine their map positions in the mouse genome (as described in “Materials and methods”). Of 9 transposons cloned from the genome, 3 were in genes (predicted transcribed regions), and all 3 were in introns.