Binding of normal human erythrocytes and CR1-deficient human erythrocytes to deletion constructs of R29-DBLα
. | . | No. rosettes* . | . | . | . | Binding efficiency, %† . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | Normal . | . | CR1-deficient . | . | Normal . | . | CR1-deficient . | . | ||||||
| Construct . | Transfection efficiency, % . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | ||||||
| R29-DBLα(C1-C14) | 33 | 120 | 105 | 40 | 25 | 36 | 39 | 11 | 10 | ||||||
| R29-DBLα(C5-C12) | 33 | 142 | 95 | 40 | 26 | 38 | 36 | 10 | 9 | ||||||
| PvRII | 60 | 217 | 144 | 204 | 153 | 56 | 55 | 53 | 50 | ||||||
| Untransfected | NA | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
. | . | No. rosettes* . | . | . | . | Binding efficiency, %† . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | Normal . | . | CR1-deficient . | . | Normal . | . | CR1-deficient . | . | ||||||
| Construct . | Transfection efficiency, % . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | ||||||
| R29-DBLα(C1-C14) | 33 | 120 | 105 | 40 | 25 | 36 | 39 | 11 | 10 | ||||||
| R29-DBLα(C5-C12) | 33 | 142 | 95 | 40 | 26 | 38 | 36 | 10 | 9 | ||||||
| PvRII | 60 | 217 | 144 | 204 | 153 | 56 | 55 | 53 | 50 | ||||||
| Untransfected | NA | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
The number of COS-7 cells with rosettes of bound erythrocytes was scored in 25 fields at × 200 magnification. Data from 2 independent experiments are reported. Transfection efficiency for experiment 1 is shown. No rosettes were seen in entire well where number of rosettes is reported as zero
The binding efficiency is calculated as follows: % binding efficiency = no. of COS cells with rosettes × 100/total no. of COS cells