Binding of normal and neuraminidase-treated human erythrocytes to deletion constructs of region F2 of EBA-175
. | . | . | No. rosettes* . | . | . | . | Binding efficiency† . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Transfection efficiency, % . | . | Normal . | . | Neu . | . | Normal . | . | Neu . | . | ||||||
| Construct . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | ||||||
| F1 + F2 | 10 | 25 | 101 | 161 | 0 | 0 | 7.0 | 11.0 | 0 | 0 | ||||||
| F2(C1-C14) | 6 | 33 | 62 | 67 | 0 | 0 | 1.5 | 7.4 | 0 | 0 | ||||||
| F2(C5-C14) | 9 | 31 | 51 | 19 | 0 | 0 | 1.7 | 1.9 | 0 | 0 | ||||||
| F2(C5-C12) | 5 | 28 | 48 | 31 | 0 | 0 | 1.0 | 3.0 | 0 | 0 | ||||||
| F2(C5-C9) | 15 | 30 | 8 | 9 | 0 | 0 | 0.5 | 1.0 | 0 | 0 | ||||||
| Untransfected | NA | NA | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
. | . | . | No. rosettes* . | . | . | . | Binding efficiency† . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Transfection efficiency, % . | . | Normal . | . | Neu . | . | Normal . | . | Neu . | . | ||||||
| Construct . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | ||||||
| F1 + F2 | 10 | 25 | 101 | 161 | 0 | 0 | 7.0 | 11.0 | 0 | 0 | ||||||
| F2(C1-C14) | 6 | 33 | 62 | 67 | 0 | 0 | 1.5 | 7.4 | 0 | 0 | ||||||
| F2(C5-C14) | 9 | 31 | 51 | 19 | 0 | 0 | 1.7 | 1.9 | 0 | 0 | ||||||
| F2(C5-C12) | 5 | 28 | 48 | 31 | 0 | 0 | 1.0 | 3.0 | 0 | 0 | ||||||
| F2(C5-C9) | 15 | 30 | 8 | 9 | 0 | 0 | 0.5 | 1.0 | 0 | 0 | ||||||
| Untransfected | NA | NA | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||||||
Neu indicates neuraminidase-treated erythrocytes; NA, not applicable.
The number of COS-7 cells with rosettes of bound erythrocytes was scored in 25 fields at × 200 magnification. Data from 2 independent experiments are reported. The number of rosettes observed was normalized for transfection efficiency of 20% for both experiments. No rosettes were observed in the entire well where number of rosettes is reported as zero
The binding efficiency is calculated as follows: % binding efficiency = no. of COS cells with rosettes × 100/total no. of COS cells